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M1428

Millipore

MIO Medium

suitable for microbiology, NutriSelect® Plus

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Synonym(s):
Motility Indole Ornithine Medium
NACRES:
NA.85

sterility

non-sterile

Quality Level

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbe id | growth & motility: suitable
microbe id | specific enzyme detection: suitable
microbiological culture: suitable

final pH

6.5±0.2 (25 °C)

application(s)

clinical testing
environmental
food and beverages
veterinary

microbiology

suitability

Escherichia coli
nonselective and differential for Enterobacter spp.
nonselective and differential for Klebsiella spp.
nonselective and differential for Proteus spp.
nonselective and differential for enterobacteriaceae

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MIO Medium suitable for microbiology, NutriSelect® Plus

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form

powder

form

powder

form

powder

form

powder

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbe id | growth & motility: suitable, microbe id | specific enzyme detection: suitable, microbiological culture: suitable

technique(s)

microbe id | growth & motility: suitable

technique(s)

microbe id | specific enzyme detection: suitable, microbiological culture: suitable

technique(s)

microbe id | specific enzyme detection: suitable, microbiological culture: suitable

final pH

6.5±0.2 (25 °C)

final pH

7.4±0.2 (25 °C)

final pH

6.0±0.2 (25 °C)

final pH

-

Application

Used for the identification of enterobacteriaceae on the basis of motility, indole production and ornithine decarboxylase activity.

Components

Ingredients (g/L)
Casein enzymatic hydrolysate, 10.00
Peptic digest of animal tissue, 10.00
Yeast extract, 3.00
L-Ornithine hydrochloride, 5.00
Dextrose, 1.00
Bromocresol purple, 0.02
Agar, 2.00

Preparation Note

Suspend 31 g of MIO Medium in 1000 ml of distilled water. Boil to dissolve the medium completely. Dispense 5 ml into each test tube. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool the tubes in an upright position.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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