M9140

Methylene blue

Dye content, ≥82%, certified by the Biological Stain Commission, powder

• Synonym(s): Basic Blue 9, 3,7-bis(Dimethylamino)phenazathionium chloride, Tetramethylthionine chloride
• Empirical Formula (Hill Notation): C₁₆H₁₈ClN₃S · xH₂O
• CAS Number: 122965-43-9
• Molecular Weight: 319.85 (anhydrous basis)
• Colour Index Number: 52015
• Beilstein/REAXYS Number: 3599847
• EC Number: 200-515-2
• MDL number: MFCD00150006
• UNSPSC Code: 12171500
• PubChem Substance ID: 24897317
• NACRES: NA.47

Properties

• grade: certified by the Biological Stain Commission
• Quality Level: 200
• form: powder
• composition: Dye content, ≥82%
• color: dark green
• solubility: H₂O: 4mg/4ml
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: CN(C)C1=CC=C2C(SC(C3=N2)=CC(C=C3)=[N+](C)C)=C1.[H]O[H].[Cl-]
• InChI: 1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1
• InChI key: WQVSELLRAGBDLX-UHFFFAOYSA-M
• Gene Information: human ... CYP1A2(1544) , GSR(2936)

Description

General description

Methylene blue is a compound that occurs as crystals or crystalline powder, having a bronze-like luster. It is soluble in water, chloroform and partially soluble in alcohol. It is a photoactive dye, which is a member of phenothiazine family.

Application

Methylene blue has been used:

• to stain vaginal cells
• for the preparation of protein aqueous ink
• to evaluate cell survival by staining

Biochem/physiol Actions

Methylene blue is used as a bacteriologic stain or dye and indicator. It mediates the inactivation of enveloped viruses in plasma, when exposed to visible light. It is an inhibitor of nitric oxide synthase and guanylate cyclase. It also plays a key role in medicine as an antimalarial agent, reducing agent in methemoglobinemia and inducer of apoptosis in cancer cells.

Suitability

Certified for use in Mallory′s phloxine-methylene blue stain; Levine & Black stain for bacteria in milk; Wright stain for blood smears.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H302
• pcodes: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves