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M9140

Sigma-Aldrich

Methylene blue

certified by the Biological Stain Commission

Synonym(s):
Basic Blue 9, 3,7-bis(Dimethylamino)phenazathionium chloride, Tetramethylthionine chloride
Empirical Formula (Hill Notation):
C16H18ClN3S · xH2O
CAS Number:
Molecular Weight:
319.85 (anhydrous basis)
Colour Index Number:
52015
Beilstein:
3599847
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

form

powder

composition

Dye content, ≥82%

solubility

H2O: 4mg/4ml

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

CN(C)C1=CC=C2C(SC(C3=N2)=CC(C=C3)=[N+](C)C)=C1.[H]O[H].[Cl-]

InChI

1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1

InChI key

WQVSELLRAGBDLX-UHFFFAOYSA-M

Gene Information

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This Item
PHR38386672028514
Methylene blue certified by the Biological Stain Commission

Sigma-Aldrich

M9140

Methylene blue

Methylene Blue certified reference material, pharmaceutical secondary standard

Supelco

PHR3838

Methylene Blue

Methylene Blue hydrate purum p.a., for microscopy (Bact., Bot., Hist., Vit.), ≥97.0% (calc. based on dry substance, AT)

Sigma-Aldrich

66720

Methylene Blue hydrate

Methylene Blue hydrate ≥95% (calc. to the dried substance)

Sigma-Aldrich

28514

Methylene Blue hydrate

grade

certified by the Biological Stain Commission

grade

certified reference material, pharmaceutical secondary standard

grade

for microscopy (Bact., Bot., Hist., Vit.), purum p.a.

grade

-

form

powder

form

-

form

-

form

powder

composition

Dye content, ≥82%

composition

-

composition

-

composition

-

solubility

H2O: 4mg/4ml

solubility

-

solubility

-

solubility

ethanol: insoluble ≤1%

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

pharmaceutical

application(s)

-

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

storage temp.

2-8°C

storage temp.

-

storage temp.

room temp

General description

Methylene blue is a compound that occurs as crystals or crystalline powder, having a bronze-like luster. It is soluble in water, chloroform and partially soluble in alcohol. It is a photoactive dye, which is a member of phenothiazine family.

Application

Methylene blue has been used:
  • to stain vaginal cells
  • for the preparation of protein aqueous ink
  • to evaluate cell survival by staining

Biochem/physiol Actions

Methylene blue is used as a bacteriologic stain or dye and indicator. It mediates the inactivation of enveloped viruses in plasma, when exposed to visible light. It is an inhibitor of nitric oxide synthase and guanylate cyclase. It also plays a key role in medicine as an antimalarial agent, reducing agent in methemoglobinemia and inducer of apoptosis in cancer cells.

Suitability

Certified for use in Mallory′s phloxine-methylene blue stain; Levine & Black stain for bacteria in milk; Wright stain for blood smears.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Acute Tox. 4 Oral

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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