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Legionella in Water PT - WP

Proficiency Testing Material


Quality Level


set of 2 ea



Featured Industry

Food and Beverages

shipped in

wet ice

storage temp.



Legionella spp.

General description

Legionella in Water - Water Pollution (WP) is produced in accordance with ISO/IEC 17043:2010. The PT portfolio allows our test components to offer significant performance data to monitor and prove competency to customers, accreditation bodies or both.

Supelco® is a global proficiency testing (PT) provider that manufactures best-in-class environmental and pharmaceutical PT products. Most water PT samples are available as concentrates or ready-to-use volumes giving the end-user flexibility in selecting samples that will fit their exact needs. Our WP program is designed to meet the quality assurance requirements, thereby ensuring the analytical competence of your laboratory.
These samples are typically provided in duplicate for use in case of lab mishap, for QC purposes or to analyze multiple methods.

Type of PT Scheme: Water analysis - Water Pollution (WP)

Matrix: Water Pollution/Wastewater

Values of analytes vary lot to lot.

All information regarding the use of Legionella in Water - Water Pollution (WP) proficiency testing standard can be found in the reporting packet which will be available to download at PT Portal.


Legionella in Water - Water Pollution (WP) proficiency testing material is designed for the quantitative determination of Legionella bacteria in wastewater samples.

Have any questions? Explore our FAQs
For more information on this product, please contact our PT Service Team.

Features and Benefits

We have a vast portfolio of environmental PT schemes with "fit-for-purpose" samples to help you meet the needs of the laboratory.

Our proficiency testing (PT) program is a benchmark to:

  • provide staff with an insight into their laboratory′s performance, to verify the accuracy and reliability of their testing process
  • provide a comparison of performance with that of other laboratories, nationally and internationally
  • test and educate staff, provide a better understanding of the impact of incorrect results and identify areas where there may be problems
  • provide an independent evaluation of laboratory performance
  • demonstrate to clients, colleagues and accreditation bodies that there is a commitment to quality


Set contains 2 sterile screw cap vials each containing a Vitroid (a small disk containing the possible organisms) and 2 - 99mL bottles of phosphate buffer.
(Please note, buffer solution may not be provided with your sample. If this causes any concern, please contact PT Service

Preparation Note


  • This PT sample is designed for the quantitative analysis of Legionella in water.
  • The PT sample is provided in duplicate as a stable dehydrated Vitroid requiring hydration. The additional sample is provided for mishaps in the lab.
  • A 100 mL of sterile hydration fluid is supplied with the PT sample. (Please note, buffer solution may not be provided with your sample. If this causes any concern, please contact PT Service This is a phosphate buffer.
  • The dehydrated Vitroid sample is stable for a time exceeding the study schedule.

General Information

  • Due to the sensitivity of microorganisms and short holding times after hydration, please read the entire instructions prior to preparation.
  • This sample is a single Vitroid and the possible concentration range is quite broad, it may be necessary to analyze the sample in a dilution series using general phosphate buffer solution.

Sample Preparation
  • Allow the capped sample vial to equilibrate to room temperature. While the sample is equilibrating to room temperature, warm the hydrating fluid in your incubator to 35 - 37 °C. This will take about 60 minutes.
  • After the hydrating fluid is warmed to 35 - 37 °C, open the sample vial and aseptically transfer the Vitroid to the hydrating fluid.

NOTE: The Vitroid is the colored disk within the cryotube. It will either be on top of cotton with opaque desiccant beads below the cotton or free moving in a cryotube that contains a desiccant tablet within the cap. The opaque desiccant beads and desiccant tablet are to keep the container dry and are not biologicals.

  • Shake well and place the sample in your incubator for 15 minutes. The hydration temperature of 35 - 37 °C is rigorous.
  • Low temperature will not assure complete hydration.
  • Higher temperatures will kill the microorganisms. After 15 minutes, remove the hydrated sample from the incubator and shake well. Inspect to assure dissolution of the Vitroid. The disk will dissolve completely.
  • This represents the sample for analysis. Dilutions are recommended to achieve a proper count but base all counts on the whole volume sample.

Storage and Stability

  • Please refer to sample label for expiration date, specific storage temperature, and specific storage conditions.
  • If storage conditions are not provided on the label, it is intended for room temperature storage

Other Notes

Are you new to Supelco® Proficiency Testing? Be sure to register your account details in the PT Portal before placing your order.
This will provide access to data entry, reporting packets and final reports. Registrations will be approved shortly after your order is placed. For further questions, write to us at

When placing your order online, please indicate your study preference under the ′your reference′ column. Available studies can be viewed at the bottom of the page.
Click here to learn more about Quick-Turn Studies (on-demand proficiency tests that are offered depending on lot availability). The Quick-Turn results are released within 48-72 hours whereas scheduled study reports are released within 21 days.

Proficiency testing samples are presented as single blind. No values for the samples are released until the testing period concludes.

Proficiency Testing Schedule (2021-2022)

WP21-2March 24 - May 7, 2021
WP21-4September 22 - November 5, 2021

WP22-2March 23 - May 6, 2022
WP22-4September 21 - November 4, 2022

Legal Information

Supelco is a registered trademark of Merck KGaA, Darmstadt, Germany
Vitroid is a trademark of Sigma-Aldrich Co. LLC



    Legionella 0.00 - 50000 CFU/mL

Storage Class Code

6.2 - Infectious substances



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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