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MTOX1000CC

Sigma-Aldrich

Caco-2 Engineered Control Cells

one vial of frozen cells

All Photos(1)

biological source

human male colorectal tissue (Source Disease: adenocarcinoma)

form

liquid

storage temp.

−196°C

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MTOX1000CC24MTOX1002MTOX1006
Sigma-Aldrich

Sigma-Aldrich

MTOX1000CC

Caco-2 Engineered Control Cells

Sigma-Aldrich

Sigma-Aldrich

MTOX1000CC24

Caco-2 Engineered Control Cells

BCRP Knockout Caco-2 Cells one vial

Sigma-Aldrich

MTOX1002

BCRP Knockout Caco-2 Cells

BCRP/MRP2 Double Knockout Caco-2 Cells one vial

Sigma-Aldrich

MTOX1006

BCRP/MRP2 Double Knockout Caco-2 Cells

storage temp.

−196°C

storage temp.

-

storage temp.

−196°C

storage temp.

−196°C

form

liquid

form

liquid

form

liquid

form

liquid

General description

The C2BBe1 cells, a sublcone of Caco-2 cells, correspond to ATCC CRL-2101. The C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years).

Application

The Engineered Control cells can be used for drug permeability assays. They may also be used as the control in conjunction with C2BBe1 Transporter Knockout cell lines for drug/transporter interaction studies. Engineered control cells have been used in the in vitro Caco-2 permeability assay. The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:

Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases

Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes

Caco-2 Transporter Knockout Cell Based Assays

Features and Benefits

The Caco-2 subclone C2BBe1 cells are ideal for transporter analysis as they express multiple transporters, are human-derived, and grow in a homogenous monolayer that forms tight junctions which is necessary for efflux ratio analysis.

Quality

Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, short terminal repeat (STR) analysis for cell line identification.

Legal Information

This product is covered under a consumable purchase agreement for one-time use only. For more information:ADME/Tox Cell Lines License

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Description
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MTOX1001P24

MDR1 Knockout Caco-2 Cells, one assay ready, 24 well plate

MTOX1002P24

BCRP Knockout Caco-2 Cells, one assay ready, 24 well plate

MTOX1003P24

MRP2 Knockout Caco-2 Cells, one assay ready, 24 well plate

MTOX1005P24

MDR1/MRP2 Double Knockout Caco-2 Cells, one assay ready, 24 well plate

MTOX1006P24

BCRP/MRP2 Double Knockout Caco-2 Cells, one assay ready, 24 well plate

MTOX1092P24

MRP1 Knockout Caco-2 Cells, one assay ready, 24 well plate

MTOX1093P24

MRP3 Knockout Caco-2 Cells, one assay ready, 24 well plate

MTOX1094P24

MRP4 Knockout Caco-2 Cells, one assay ready, 24 well plate

MTOX1095P24

MRP5 Knockout Caco-2 Cells, one assay ready, 24 well plate

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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P Artursson
Journal of pharmaceutical sciences, 79(6), 476-482 (1990-06-01)
A human intestinal cell line, Caco-2, was used as a model to study the passive diffusion of drugs across intestinal epithelium. The cells formed continuous monolayers when grown on permeable filters of polycarbonate. After 10 days in culture, the monolayers
Mark I Kaldas et al.
The Journal of pharmacy and pharmacology, 55(3), 307-312 (2003-05-02)
Resveratrol is a dietary constituent suggested to have protective effects against cancer as well as cardiovascular disease. The purpose of the study was to learn whether this agent could be absorbed in man and enter the systemic circulation. This was
M J Briske-Anderson et al.
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.), 214(3), 248-257 (1997-03-01)
The Caco-2 cell line is used by many investigators as a model of the intestinal epithelium to study nutrient uptake and transport. Our goal was to create an awareness of inherent variabilities in the Caco-2 cell line which may influence
S Yee
Pharmaceutical research, 14(6), 763-766 (1997-06-01)
To evaluate and optimize the use of Caco-2 cell monolayers to predict the in vivo absorption of a broad range of compounds in man. Caco-2 cells are derived from human adenocarcinoma colon cells and spontaneously differentiate when grown on porous
V Pade et al.
Journal of pharmaceutical sciences, 87(12), 1604-1607 (1999-04-03)
The objective of this investigation was to establish a relationship between drug permeability and solubility in vitro and the extent of drug absorption in humans. We selected drugs with varying permeabilities and solubilities with the aim of establishing a relationship
View All Related Papers

Articles

The Role of Intestinal Efflux Transporters In Drug Absorption

We presents an article on The Role of Intestinal Efflux Transporters In Drug Absorption.

Caco-2 Intestinal Drug Transporter Models for More Predictive Drug Absorption

Utilize these Caco-2 cell based assay tools for screening small molecule drug compounds prior to clinical studies and submission to regulatory agencies.

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