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N1636

Sigma-Aldrich

β-Nicotinamide adenine dinucleotide hydrate

purified by column chromatography, ≥99%

Synonym(s):
Cozymase, β-NAD, β-DPN, DPN, Diphosphopyridine nucleotide, NAD, Coenzyme 1, Nadide
Empirical Formula (Hill Notation):
C21H27N7O14P2 · xH2O
CAS Number:
Molecular Weight:
663.43 (anhydrous basis)
EC Number:
MDL number:
eCl@ss:
39200202
PubChem Substance ID:
NACRES:
NA.55

Quality Level

assay

≥99%

form

crystalline powder

color

white

storage temp.

−20°C

SMILES string

NC1=NC=NC2=C1N=CN2.O[C@@H]3[C@@H](COP(O)(OP(OC[C@@H](O4)[C@@H](O)[C@@H](O)[C@H]4[N+]5=CC=CC(C(N)=O)=C5)([O-])=O)=O)OC[C@@H]3O

InChI

1S/C21H27N7O14P2/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(32)14(30)11(41-21)6-39-44(36,37)42-43(34,35)38-5-10-13(29)15(31)20(40-10)27-3-1-2-9(4-27)18(23)33/h1-4,7-8,10-11,13-16,20-21,29-32H,5-6H2,(H5-,22,23,24,25,33,34,35,36,37)/t10-,11-,13-,14-,15-,16-,20-,21-/m1/s1

InChI key

BAWFJGJZGIEFAR-NNYOXOHSSA-N

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General description

β-Nicotinamide adenine dinucleotide (NAD) is an electron carrier and a cofactor. It is ubiquitously found in the living system. NAD+ contains an adenylic acid and a nicotinamide-5′-ribonucleotide group linked together by a pyrophosphate moiety. In NAD+ complexes, the enzyme-cofactor interactions are highly conserved.

Application

β-Nicotinamide adenine dinucleotide hydrate has been used:
  • to measure adenosine triphosphate (ATP) synthesis rates (kinetics measurements)
  • in the reaction buffer for pyruvate kinase (PK) activity assay
  • as a component of master mix for a high throughput screening

Packaging

1 g in poly bottle
25, 100, 250 mg in poly bottle
Packaged by solid weight.

Biochem/physiol Actions

β-Nicotinamide adenine dinucleotide (NAD) cycles between the oxidized (NAD+) and reduced (NADH) forms to maintain a redox balance necessary for continued cell growth. It significantly participates in enzyme-catalyzed oxido-reduction processes and many genetic processes. It is also involved in microbial catabolism.
Electron acceptor

Other Notes

Crystalline NAD contains less than 0.1% (w/w) residual organic solvent, is less hygroscopic and electrostatic, and is more stable at ambient temperatures than amorphous NAD.
This is the common form of NAD.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

More Documents

Quotes and Ordering

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Isolation of Tomato Fruit Chromoplasts and Determination of ATP Levels
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Bio-protocol, 4(15), e1192-e1192 (2014)
Experimental and theoretical electron density studies in large molecules: NAD+, beta-nicotinamide adenine dinucleotide
Guillot B, et al.
The Journal of Physical Chemistry B, 107(34), 9109-9121 (2003)
A Mechanism of Adsorption of beta-Nicotinamide Adenine Dinucleotide on Graphene Sheets: Experiment and Theory
Pumera M, et al.
Chemistry (Weinheim An Der Bergstrasse, Germany), 15(41), 10851-10856 (2009)

Articles

Electron Transport

Learn about the four membrane-bound protein complexes that make up the electron transport chain metabolic pathway supplying energy as ATP for cellular respiration.

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