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β-Nicotinamide adenine dinucleotide hydrate

purified by column chromatography, ≥99%

Cozymase, β-NAD, β-DPN, DPN, Diphosphopyridine nucleotide, NAD, Coenzyme 1, Nadide
Empirical Formula (Hill Notation):
C21H27N7O14P2 · xH2O
CAS Number:
Molecular Weight:
663.43 (anhydrous basis)
EC Number:
MDL number:
PubChem Substance ID:

Quality Level




crystalline powder



storage temp.


SMILES string




InChI key


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General description

β-Nicotinamide adenine dinucleotide (NAD) is an electron carrier and a cofactor. It is ubiquitously found in the living system. NAD+ contains an adenylic acid and a nicotinamide-5′-ribonucleotide group linked together by a pyrophosphate moiety. In NAD+ complexes, the enzyme-cofactor interactions are highly conserved.


β-Nicotinamide adenine dinucleotide hydrate has been used:
  • to measure adenosine triphosphate (ATP) synthesis rates (kinetics measurements)
  • in the reaction buffer for pyruvate kinase (PK) activity assay
  • as a component of master mix for a high throughput screening


1 g in poly bottle
25, 100, 250 mg in poly bottle
Packaged by solid weight.

Biochem/physiol Actions

β-Nicotinamide adenine dinucleotide (NAD) cycles between the oxidized (NAD+) and reduced (NADH) forms to maintain a redox balance necessary for continued cell growth. It significantly participates in enzyme-catalyzed oxido-reduction processes and many genetic processes. It is also involved in microbial catabolism.
Electron acceptor

Other Notes

Crystalline NAD contains less than 0.1% (w/w) residual organic solvent, is less hygroscopic and electrostatic, and is more stable at ambient temperatures than amorphous NAD.
This is the common form of NAD.

Storage Class Code

13 - Non Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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More Documents

Quotes and Ordering

Identification and characterization of novel sirtuin inhibitor scaffolds
Sanders BD, et al.
Bioorganic & Medicinal Chemistry, 17(19), 7031-7041 (2009)
Techniques to monitor glycolysis
Test, 542(15), 91-114 (2014)
Isolation of Tomato Fruit Chromoplasts and Determination of ATP Levels
Hernandez GF, et al.
Bio-protocol, 4(15), e1192-e1192 (2014)
Experimental and theoretical electron density studies in large molecules: NAD+, beta-nicotinamide adenine dinucleotide
Guillot B, et al.
The Journal of Physical Chemistry B, 107(34), 9109-9121 (2003)
A Mechanism of Adsorption of beta-Nicotinamide Adenine Dinucleotide on Graphene Sheets: Experiment and Theory
Pumera M, et al.
Chemistry (Weinheim An Der Bergstrasse, Germany), 15(41), 10851-10856 (2009)


Electron Transport

Learn about the four membrane-bound protein complexes that make up the electron transport chain metabolic pathway supplying energy as ATP for cellular respiration.

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