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N5514

Sigma-Aldrich

Nitro Blue Tetrazolium

tablet

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Synonym(s):
Nitrotetrazolium Blue Chloride
CAS Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

form

tablet

Quality Level

color

yellow

pH

4.5-5.5

solubility

soluble, clear, yellow

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8

InChI

1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2

InChI key

FSVCQIDHPKZJSO-UHFFFAOYSA-L

General description

Nitro Blue Tetrazolium is a tetrazole compound with two tetrazole rings.

Application

Nitro Blue Tetrazolium has been used:
  • to measure superoxide radicals
  • in lactate dehydrogenase quantification assay
  • for blot development in western blotting analysis
  • in NADPH-d histochemistry
  • as a reagent to analyse the properties of murine intestinal mucins by electrophoresis and histology
  • in immunohistochemistry

Quantity

Contains 10 mg substrate per tablet.

pictograms

CorrosionExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Nitrotetrazolium blue chloride as a novel corrosion inhibitor of steel in sulfuric acid solution
Deng S, et al
Corrosion Science, 52(11), 3840-3846 (2010)
Salmonella Serovars and Vaccination Effect on the Immune Responses of Male and Female Layers
Chen Kl, et al.
The journal of poultry science, 53(4), 318-328 (2016)
Analyzing the Properties of Murine Intestinal Mucins by Electrophoresis and Histology
Wang R and Hasnain SZ
PLoS Pathogens (2017)
Night light-adaptation strategies for photosynthetic apparatus in yellow-poplar (Liriodendron tulipifera L.) exposed to artificial night lighting
Kwak MJ, et al.
Forests, 9(2), 74-74 (2018)
Luttmann W
Immunology, The Experimenter Series (2006)

Articles

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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