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N6876

Sigma-Aldrich

Nitrotetrazolium Blue chloride

≥90.0% (HPLC)

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Synonym(s):
2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride, 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, NBT, Nitro BT
Linear Formula:
C40H30N10O6 · 2Cl
CAS Number:
Molecular Weight:
817.64
Beilstein/REAXYS Number:
4115923
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

assay

≥90.0% (HPLC)

form

powder

impurities

≤10% solvent

solubility

H2O: 10 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8

InChI

1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2

InChI key

FSVCQIDHPKZJSO-UHFFFAOYSA-L

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1 of 4

This Item
T437588190N6639
vibrant-m

N6876

Nitrotetrazolium Blue chloride

vibrant-m

T4375

Tetrazolium Blue Chloride

vibrant-m

88190

Tetrazolium Blue Chloride

vibrant-m

N6639

Nitrotetrazolium Blue chloride

assay

≥90.0% (HPLC)

assay

-

assay

≥90% (T)

assay

>90%

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

storage temp.

2-8°C

storage temp.

room temp

storage temp.

-

storage temp.

2-8°C

form

powder

form

powder

form

powder

form

powder

impurities

≤10% solvent

impurities

-

impurities

-

impurities

-

Application

  • Nitrotetrazolium Blue chloride has been used to detect the generation of oxygen metabolites in cells.
  • It has been used as a substrate for western blot detection.
  • It has been used for the staining of histological tissue sections.

Biochem/physiol Actions

Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridized to its target.

Substrates

Substrate for dehydrogenases and other oxidases.

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pictograms

Exclamation markHealth hazard

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Proceedings of the National Academy of Sciences of the USA, 98, 11444-11444 (2001)
Expression of the endothelial lipase gene in murine embryos and reproductive organs.
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Journal of Lipid Research, 46, 439-439 (2005)
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Articles

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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