NIST972A

Vitamin D metabolites in frozen human serum

NIST^® SRM^® 972a

• NACRES: NA.24
• UNSPSC Code: 41116107

Properties

• grade: certified reference material
• Quality Level: 100
• form: liquid
• packaging: pkg of 4 x 1 mL
• manufacturer/tradename: NIST^®
• technique(s): mass spectrometry (MS): suitable
• application(s): clinical testing
• format: matrix material

Description

General description

Determining total 25-hydroxy vitamin D [25(OH)D] concentration in serum, the sum of 25-hydroxyvitamin D₂ [25(OH)D₂] and 25-hydroxyvitamin D₃ [25(OH)D₃] is generally considered a reliable indicator of vitamin D status. Measurement of 24R, 25(OH)₂D₃ in serum is considered a catabolism marker and an indicator of kidney disease. A unit of SRM 972a contains 1 mL of four vials (levels 1 through 4) of frozen serum with different concentration levels of 25-hydroxyvitamin D [25(OH)D] and 24R, 25-dihydroxy vitamin D₃ [24R,25(OH)₂D₃].

NIST972A_Cert

NIST972A_SDS

Application

This vitamin D metabolite standard reference material (SRM) is intended for use as an accuracy control in the evaluation of methods for determining the amount-of-substance concentration of vitamin D metabolites in human serum. This SRM can also be used as a quality assurance tool for assigning values to in-house control materials for these constituents.

Vitamin D metabolites in frozen human serum has been used as a reference material for the calibration and standardization of 25-hydroxyvitamin D (25(OH)D) from dried blood spot samples by liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Biochem/physiol Actions

Vitamin D known as cholecalciferol exists as vitamin D₃. It is taken in the diet through fortified dairy products and fish oils. It is synthesized in the skin from 7-dehydrocholesterol by ultraviolet irradiation. The biologically active form of vitamin D, 25(OH)₂D₃, is essential for mineral metabolism, and for other physiological functions like inhibition of growth of cancer cells and protection against certain immune mediated disorders.

Preparation Note

• The vials should be stored in the dark at a temperature between –20 °C and –80 °C.
• Before use, the vial/s must be thawed at room temperature for about 30 mins in subdued light.

Other Notes

• Information on expiry, storage, and usage is provided in the NIST certificate.
• Notes on Biomaterials, disposal, and transport are available on NIST MSDS.

Legal Information

NIST is a registered trademark of National Institute of Standards and Technology

SRM is a registered trademark of National Institute of Standards and Technology

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable