O0625

Oil Red O

certified by the Biological Stain Commission

• Synonym(s): 1-([4-(Xylylazo)xylyl]azo)-2-naphthol, 1-[2,5-Dimethyl-4-(2,5-dimethylphenylazo)phenylazo]-2-naphthol, Solvent Red 27
• Empirical Formula (Hill Notation): C₂₆H₂₄N₄O
• CAS Number: 1320-06-5
• Molecular Weight: 408.49
• Colour Index Number: 26125
• EC Number: 215-295-3
• MDL number: MFCD00003898
• PubChem Substance ID: 24897931
• NACRES: NA.47

Properties

• grade: certified by the Biological Stain Commission
• Quality Level: 200
• form: powder
• composition: Dye content, ≥75%
• mp: 120 °C (dec.) (lit.)
• solubility: chloroform/ethanol (1:1): 1 mg/mL
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: Cc1ccc(C)c(c1)\N=N\c2cc(C)c(cc2C)\N=N/c3c(O)ccc4ccccc34
• InChI: 1S/C26H24N4O/c1-16-9-10-17(2)22(13-16)27-28-23-14-19(4)24(15-18(23)3)29-30-26-21-8-6-5-7-20(21)11-12-25(26)31/h5-15,31H,1-4H3
• InChI key: NPGIHFRTRXVWOY-UHFFFAOYSA-N

Description

General description

Oil Red O is a lysochrome.[1] It is used to stain and visualise general localization of fats.[2]

Oil Red O stains lipid materials and the lipids take red-orange color. It also marks vacuoles, particularly observed in ALL-L3 (acute lymphoblastic leukemia) or Burkitt′s lymphoma/leukemia. Oil Red O staining is done on fresh samples, since alcohol fixation removes lipid.[3][4]

Application

Oil Red O has been used for the staining of lipid droplets and lipid-containing vacuoles in cells.[5][6]

Oil Red O has been used:

• for staining liver sections in histological analysis[7][8][9]
• for the quantification of lipids in cells[10][11][12]
• to stain aorta to examine atherosclerotic lesions[13]

Packaging

25, 100 g in glass bottle

Suitability

Certified for use as a fat stain in Churukian′s modification of the method of Lillie and Asburn.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves