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O0625

Sigma-Aldrich

Oil Red O

certified by the Biological Stain Commission

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Synonym(s):
1-([4-(Xylylazo)xylyl]azo)-2-naphthol, 1-[2,5-Dimethyl-4-(2,5-dimethylphenylazo)phenylazo]-2-naphthol, Solvent Red 27
Empirical Formula (Hill Notation):
C26H24N4O
CAS Number:
Molecular Weight:
408.49
Colour Index Number:
26125
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

form

powder

composition

Dye content, ≥75%

mp

120 °C (dec.) (lit.)

solubility

chloroform/ethanol (1:1): 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cc1ccc(C)c(c1)\N=N\c2cc(C)c(cc2C)\N=N/c3c(O)ccc4ccccc34

InChI

1S/C26H24N4O/c1-16-9-10-17(2)22(13-16)27-28-23-14-19(4)24(15-18(23)3)29-30-26-21-8-6-5-7-20(21)11-12-25(26)31/h5-15,31H,1-4H3

InChI key

NPGIHFRTRXVWOY-UHFFFAOYSA-N

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1 of 4

This Item
O1391O1516S4131
Oil Red O certified by the Biological Stain Commission

Sigma-Aldrich

O0625

Oil Red O

Oil Red O solution 0.5% in isopropanol

Sigma-Aldrich

O1391

Oil Red O solution

Oil Red O solution 0.5% in propylene glycol

Sigma-Aldrich

O1516

Oil Red O solution

Sudan III Technical grade

Sigma-Aldrich

S4131

Sudan III

form

powder

form

liquid

form

liquid

form

powder

composition

Dye content, ≥75%

composition

-

composition

-

composition

Dye content, ≥85%

mp

120 °C (dec.) (lit.)

mp

-

mp

-

mp

199 °C (dec.) (lit.)

solubility

chloroform/ethanol (1:1): 1 mg/mL

solubility

-

solubility

-

solubility

chloroform: 1 mg/mL, clear, red

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

General description

Oil Red O is a lysochrome. It is used to stain and visualise general localization of fats.
Oil Red O stains lipid materials and the lipids take red-orange color. It also marks vacuoles, particularly observed in ALL-L3 (acute lymphoblastic leukemia) or Burkitt′s lymphoma/leukemia. Oil Red O staining is done on fresh samples, since alcohol fixation removes lipid.

Application

Oil Red O has been used for the staining of lipid droplets and lipid-containing vacuoles in cells.
Oil Red O has been used:
  • for staining liver sections in histological analysis
  • for the quantification of lipids in cells
  • to stain aorta to examine atherosclerotic lesions

Suitability

Certified for use as a fat stain in Churukian′s modification of the method of Lillie and Asburn.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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25G
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Theory and Practice of Histological Techniques (2008)
Ablation of Myeloid ADK (Adenosine Kinase) Epigenetically Suppresses Atherosclerosis in ApoE-/-(Apolipoprotein E Deficient) Mice
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