If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
P0532
Phthaldialdehyde Reagent
Solution Complete
Synonym(s):
OPA
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 5 mL | Available to ship TODAYfromMILWAUKEE | $45.10 | |
| 50 mL | Available to ship TODAYfromMILWAUKEE | $87.00 | |
| 250 mL | Available to ship TODAYfromMILWAUKEE | $263.00 |
About This Item
Quality Level
storage temp.
2-8°C
SMILES string
[H]C(=O)c1ccccc1C([H])=O
InChI
1S/C8H6O2/c9-5-7-3-1-2-4-8(7)6-10/h1-6H
InChI key
ZWLUXSQADUDCSB-UHFFFAOYSA-N
Application
Other Notes
1 of 1
This Item | |||
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| storage temp. 2-8°C | storage temp. 2-30°C | storage temp. 2-8°C | storage temp. 2-8°C |
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signalword
Danger
Hazard Classifications
Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Repr. 1B - Skin Corr. 1B - Skin Sens. 1
wgk
WGK 3
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Related Content
Instructions
Global Trade Item Number
| SKU | GTIN |
|---|---|
| P0532-250ML | 04061837841255 |
| P0532-5ML | 04061834304302 |
| P0532-50ML | 04061834304296 |
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How can I determine the shelf life / expiration / retest date of this product?
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I am having trouble applying this reagent to my GelMa substitution degree detection assay. How should I apply this reagent to 96-well plates containing gelatin as a control?
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The Phthaldialdehyde reagent needs to be freshly prepared according to the protocol, as the material can be sensitive to degradation. In the 96-well plate, control wells should be set up with gelatin. A series of wells with varying concentrations of gelatin can be used for comparison against the samples. A specific volume of the GelMa sample should be added to the designated wells. For control wells with gelatin, the same volume of gelatin solution should be added. After incubation, the prepared Phthaldialdehyde reagent should be added to each well. The volume should be consistent across all wells (commonly around 50-100 µL). The contents of the wells should be gently mixed by pipetting up and down or using a plate shaker, if available. The reaction will develop over the next 30-60 minutes, and absorbance can be measured using a plate reader at the appropriate wavelength (often around 340 nm) to quantify the degree of substitution based on the intensity of the color developed.
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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1) What type of container this reagent stored at when you supply? 2) After I use for the first time, can you pls explain about proper storing? Should I purge inside the container with N2 gas? or just keep the bottle in N2 environment?
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1. Phthaldialdehyde Reagent is provided in an amber glass bottle.
2. Work under a fume hood. Do not inhale substance/mixture. Avoid generation of vapours/aerosols. Store in a tightly closed. Keep it in a well-ventilated place.
3. To store under inert gas, open the cap and then direct a gentle stream of nitrogen or argon gas down into the vial for a few seconds. Closely tightly and store at t 2–8 °C.Helpful?
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Can you please explain about the stability of the reagent? When I open the bottle during derivatization time, should I maintain inert environment too?
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Special care should be taken to ensure the product is maintained in an inert atmosphere (nitrogen or argon) at 2–8 °C since improper storage or handling conditions may render the solution ineffective. Please review the product information sheet for more details.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/326/300/p0532dat.pdfHelpful?
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이 시약이 HPLC 에 사용될 수 있나요 ?? 등급이 따로 기재되어 있지 않아서 문의드립니다.
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While the grade of the Phthaldialdehyde Reagent is not listed, it is used for the pre-column derivatization of primary amines and amino acids for HPLC analysis. So this reagent can be used in HPLC analysis.
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I would like to ask if this is a readu-to-use reagent for amino acid derivatization purposes or if it needs further mixing with other reagents, e.g. mercaptoethanol and so on.
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This product already contains mercaptoethanol and other reagents, as can be seen on the SDS. It is ready to use. Please see the product information sheet for more information around storage and use: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/326/300/p0532dat.pdf.
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