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Key Documents

P0914

Sigma-Aldrich

Protein standard

Micro Standard, liquid

Synonym(s):

Albumin from bovine serum, protein standard

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.27
Pricing and availability is not currently available.

biological source

bovine

Quality Level

form

liquid

contains

0.05% sodium azide as preservative

packaging

ampule of 1 mL

concentration

0.98—1.02 mg protein/mL

technique(s)

ELISA: suitable

color

clear colorless

UniProt accession no.

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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1 of 4

This Item
820464820452820456
technique(s)

ELISA: suitable

technique(s)

ELISA: suitable, enzyme immunoassay: suitable

technique(s)

ELISA: suitable, enzyme immunoassay: suitable

technique(s)

ELISA: suitable, enzyme immunoassay: suitable

biological source

bovine

biological source

-

biological source

-

biological source

-

Quality Level

200

Quality Level

300

Quality Level

300

Quality Level

300

form

liquid

form

solution (30%)

form

lyophilized powder, solution (30%)

form

lyophilized powder

packaging

ampule of 1 mL

packaging

pkg of 50 mL

packaging

pkg of 1 kg

packaging

pkg of 5 kg

Gene Information

bovine ... ALB(280717)

Gene Information

-

Gene Information

-

Gene Information

-

Application

Concentrated solution for all protein assays.
Protein standard has been used:

  • as a standard in the quantification of protein release by bicinchoninic acid assay[1]
  • as a standard in the quantification of urea by Bradfordassay[1]
  • as a molecular size marker for the quantification of protein by native polyacrylamide gel electrophoresis[2]
Protein standard for all protein assays. The sealed ampules provide assurance that the concentration will be correct with every assay.

Packaging

Sealed ampules

Other Notes

1 mg bovine serum albumin/ml in 0.15 M NaCl

Suitability

This product is commonly used for protein determination by the Bicinchoninic Acid (BCA) method.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Certificates of Analysis (COA)

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Questions

1–4 of 4 Questions  
  1. Can bsa P0914-5AMP be diluted in tbs-t to make block buffer for electrophoresis?

    1 answer
    1. This product is a 1 mg/mL solution of BSA in 0.15 M NaCl. There are no anticipated problems with further diluting in Tris-based or other aqueous buffer solutions for blocking purposes.

      Helpful?

  2. What is the shipping temperature?

    1 answer
    1. The item is shipped at ambient temperature. A transit time of up to 2 weeks will not impact the quality of this material. Upon delivery, this product should be stored at 2-8°C.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. Do you have any information on the traceability to a recognized protein standard, such as NIST? Or is the concentration derived from accepted values of natural physical constants?

    1 answer
    1. The concentration of each lot of the protein standard, Product P0914, is determined by UV measurements. Specifically, given a known extinction coefficient (E) and a measured absorbance (A), and assuming a standard pathlength of 1 cm, the concentration (c) can be determined by Beer's Law (c = A/E). The assay result is derived from an accepted value of a natural physical constant. Specifically, the E1% of 6.67 at approximately 279 nm is a well-accepted value, originally published in J. Am. Chem. Soc., 78, 3656-3660 (1956).

      Helpful?

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