MilliporeSigma
All Photos(1)

Documents

P1379

Sigma-Aldrich

TWEEN® 20

viscous liquid

Sign Into View Organizational & Contract Pricing

Synonym(s):
Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate
CAS Number:
MDL number:
NACRES:
NA.21

description

non-ionic

Quality Level

assay

>40% (GC)

form

viscous liquid

mol wt

~1228

composition

lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids)

concentration

≥40.0% (GC)

technique(s)

DNA extraction: suitable
RNA extraction: suitable
cell culture | mammalian: suitable
protein purification: suitable

impurities

≤3.0% water

refractive index

n20/D 1.468 (lit.)

CMC

0.06 mM (20-25°C)

acid number

≤2.2 mg/g

hydroxyl value

96‑108 mg/g

transition temp

cloud point 76 °C

density

1.095 g/mL at 25 °C (lit.)

HLB

16.7

suitability

complies for IR spectroscopy

SMILES string

CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO

InChI

1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3

InChI key

HMFKFHLTUCJZJO-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
P8341P65859480-OP
vibrant-m

P1379

TWEEN® 20

vibrant-m

P8341

TWEEN® 20

vibrant-m

P6585

TWEEN® 20

form

viscous liquid

form

viscous liquid

form

viscous liquid

form

viscous liquid

technique(s)

DNA extraction: suitable, cell culture | mammalian: suitable, RNA extraction: suitable, protein purification: suitable

technique(s)

-

technique(s)

-

technique(s)

-

suitability

complies for IR spectroscopy

suitability

-

suitability

-

suitability

-

HLB

16.7

HLB

16.7

HLB

16.7

HLB

-

mol wt

~1228

mol wt

~1228

mol wt

~1228

mol wt

~1228

General description

Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.
Tween® 20 is a nonionic surfactant and detergent widely applied in biochemical and life science research. Its predominant use involves the solubilization of membrane proteins during the isolation of membrane-protein complexes. Moreover, it serves as a component in various washing, blocking, dilution, and stabilization buffers. Additionally, it functions as a reagent for immunohistochemistry, encompassing techniques like Western blotting and ELISAs. In these applications, Tween® 20 plays crucial roles such as preventing non-specific antibody binding, minimizing background staining, and ensuring the even distribution of reagents.

Surfactants, possessing both hydrophilic and hydrophobic components, fall into two main categories: ionic, which ionize in water (anionic, cationic, or ampholytic), and nonionic, which do not ionize. The discussed product is a nonionic surfactant with diverse applications such as emulsification, dispersion, solubilization, and detergent use. Additionally, it can serve as a solubilizer for membrane proteins.

Application

TWEEN 20 has been used as a component of:
  • washing buffer and blocking buffer in Western blotting
  • blocking buffer in immunohistochemistry
  • washing buffer in ELISA (enzyme linked immunosorbent assay)
  • reaction mixture in PCR (polymerase chain reaction)
TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.
Non-ionic detergent

Features and Benefits

  • Highly versatile surfactant for your life science and biochemical research
  • Excellent solubilising, dispersing and emulsifying agent
  • Suitable for DNA & RNA extraction, mammalian cell culture, and protein purification

Packaging

P1379-1KT:
Each kit contains 3 x 100ML samples, each sample from a uniquely manufactured lot.

Preparation Note

TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

527.0 °F - Pensky-Martens closed cup

flash_point_c

275 °C - Pensky-Martens closed cup

ppe

Eyeshields, Gloves


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 6

1 of 6

Sigma-Aldrich

Sigma-Aldrich

P1504

TWEEN® 40

Triton™ X-100 laboratory grade

Sigma-Aldrich

X100

Triton X-100

Polysorbate 20 tested according to Ph. Eur.

Sigma-Aldrich

44112

Polysorbate 20

TWEEN® 20 (Polysorbate) EMPROVE® ESSENTIAL, Ph. Eur., JPE, NF

SAFC

8.17072

TWEEN® 20 (Polysorbate)

Polysorbate 20

Sigma-Aldrich

W291501

Polysorbate 20

Polysorbates 20 and 80 used in the formulation of protein biotherapeutics: structure and degradation pathways.
Kerwin BA
J. Pharm. Pharm. Sci., 97(8), 2924-2935 (2008)
Ethylene and carbon dioxide production by developing strawberries show a correlative pattern that is indicative of ripening climacteric fruit.
Lannetta PM et al
Physiologia Plantarum, 127.2, 247-259 (2006)
Gene expression and immune response kinetics using electroporation-mediated DNA delivery to muscle.
Gr?nevik E et al
The journal of gene medicine, 7(2), 218-227 (2005)
Thomas Beuchert Kallehauge et al.
Scientific reports, 7, 40388-40388 (2017-01-17)
Recombinant protein production coopts the host cell machinery to provide high protein yields of industrial enzymes or biotherapeutics. However, since protein translation is energetically expensive and tightly controlled, it is unclear if highly expressed recombinant genes are translated as efficiently
Jennifer Somerfield et al.
Journal of immunology (Baltimore, Md. : 1950), 185(1), 763-768 (2010-06-04)
Biological therapies, even humanized mAbs, may induce antiglobulin responses that impair efficacy. We tested a novel strategy to induce tolerance to a therapeutic mAb. Twenty patients with relapsing-remitting multiple sclerosis received an initial cycle of alemtuzumab (Campath-1H), up to 120

Articles

Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.

Protocols

This page shows how to solubilize membrane proteins with products from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service