P2759

Phloxine B

antibacterial fluorescent dye

• Synonym(s): 2′,4′,5′,7′-Tetrabromo-4,5,6,7-tetrachlorofluorescein disodium salt, Acid Red 92, Cyanosine, D&C;Red No. 28, Eosin 10B
• Empirical Formula (Hill Notation): C₂₀H₂Br₄Cl₄Na₂O₅
• CAS Number: 18472-87-2
• Molecular Weight: 829.63
• Colour Index Number: 45410
• Beilstein: 3887100
• EC Number: 242-355-6
• MDL number: MFCD00070627
• PubChem Substance ID: 24898359

Properties

• Quality Level: 200
• form: powder
• solubility: water: 1 mg/mL, clear, red
• εmax: 1150-1300 at 546-550 nm in 50% ethanol
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].[Na+].[O-]c1c(Br)cc2c(Oc3c(Br)c([O-])c(Br)cc3C24OC(=O)c5c(Cl)c(Cl)c(Cl)c(Cl)c45)c1Br
• InChI: 1S/C20H4Br4Cl4O5.2Na/c21-5-1-3-17(9(23)15(5)29)32-18-4(2-6(22)16(30)10(18)24)20(3)8-7(19(31)33-20)11(25)13(27)14(28)12(8)26;;/h1-2,29-30H;;/q;2*+1/p-2
• Inchi Key: OOYIOIOOWUGAHD-UHFFFAOYSA-L

Description

Application

Phloxine B has been used to trace the uptake of double stranded RNA by green peach aphids. It has also been used to stain yeast strains.

Biochem/physiol Actions

Phloxine B has been demonstrated as an alternative to Gram staining. It was found to inhibit the growth of methicillin-resistant Staphylococcus aureus. Phloxine B stimulated and inhibited the channel activity of wild-type cystic fibrosis transmembrane conductance regulator (CFTR). It has antibacterial properties. Phloxine B staining can be used to distinguish between gram-positive and gram-negative bacteria.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves