MilliporeSigma
All Photos(3)

P2759

Sigma-Aldrich

Phloxine B

antibacterial fluorescent dye

Synonym(s):
D&C;Red No. 28, Acid Red 92, Eosin 10B, Cyanosine, 2′,4′,5′,7′-Tetrabromo-4,5,6,7-tetrachlorofluorescein disodium salt
Empirical Formula (Hill Notation):
C20H2Br4Cl4Na2O5
CAS Number:
Molecular Weight:
829.63
Colour Index Number:
45410
Beilstein:
3887100
EC Number:
MDL number:
PubChem Substance ID:

Quality Level

form

powder

solubility

water: 1 mg/mL, clear, red

εmax

1150-1300 at 546-550 nm in 50% ethanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[O-]c1c(Br)cc2c(Oc3c(Br)c([O-])c(Br)cc3C24OC(=O)c5c(Cl)c(Cl)c(Cl)c(Cl)c45)c1Br

InChI

1S/C20H4Br4Cl4O5.2Na/c21-5-1-3-17(9(23)15(5)29)32-18-4(2-6(22)16(30)10(18)24)20(3)8-7(19(31)33-20)11(25)13(27)14(28)12(8)26;;/h1-2,29-30H;;/q;2*+1/p-2

InChI key

OOYIOIOOWUGAHD-UHFFFAOYSA-L

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Application

Phloxine B has been used to trace the uptake of double stranded RNA by green peach aphids. It has also been used to stain yeast strains.

Packaging

25, 100 g in glass bottle

Biochem/physiol Actions

Phloxine B has been demonstrated as an alternative to Gram staining. It was found to inhibit the growth of methicillin-resistant Staphylococcus aureus. Phloxine B stimulated and inhibited the channel activity of wild-type cystic fibrosis transmembrane conductance regulator (CFTR). It has antibacterial properties. Phloxine B staining can be used to distinguish between gram-positive and gram-negative bacteria.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Phloxine B, a versatile bacterial stain
Reuven Rrasooly
Pathogens and Disease (2007)
Advances in measuring lifespan in the yeast Saccharomyces cerevisiae
Nadege Minios
Proceedings of the National Academy of Sciences of the USA (2004)
Using Vital Dyes to Trace Uptake of dsRNA by Green Peach Aphid Allows Effective Assessment of Target Gene Knockdown
International Journal of Molecular Sciences (2017)
Phloxine B Interacts with the Cystic Fibrosis Transmembrane Conductance Regulator at Multiple Sites to Modulate Channel Activity*210
Zhiwei Cai and David N. Sheppard
The Journal of Biological Chemistry (2002)
Ellyn E Ritchie et al.
Chemosphere, 90(7), 2129-2135 (2012-12-06)
Soil eco-toxicity testing was conducted in support of Canada's Chemical Management Plan (CMP) to fill data gaps for organic chemicals known to primarily partition to soil, and of which the persistence and inherent toxicity are uncertain. Two compounds representative of

Protocols

HPLC Analysis of Synthetic Food Dyes on Ascentis® Express C18

Separation of Tartrazine; Amaranth; Indigo carmine; New Coccine; Sunset Yellow FCF; Allura Red AC; Fast Green FCF; Erioglaucine disodium salt; Erythrosin B sodium salt; Phloxine B; Rose bengal

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