P3504

Sigma-Aldrich

Ponceau S

BioReagent, suitable for electrophoresis

Synonym(s):
3-Hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2,7-naphthalenedisulfonic acid sodium salt
Empirical Formula (Hill Notation):
C22H12N4Na4O13S4
CAS Number:
Molecular Weight:
760.57
Colour Index Number:
27195
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47
Pricing and availability is not currently available.

Quality Level

product line

BioReagent

form

powder

application(s)

electrophoresis: suitable
hematology: suitable
histology: suitable

solubility

H2O: 1 mg/mL

ε (extinction coefficient)

≥330000 at 518-522 nm in deionized water at 0.015 g/L

Featured Industry

Diagnostic Assay Manufacturing

storage temp.

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c4ccc(cc4cc1S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;;;

InChI key

KQHKSGRIBYJYFX-WNDMICSESA-J

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General description

Ponceau S is the sodium salt of a diazo dye. It is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. The stain is reversed with water washes and aids in subsequent immunological detection.
The following are two common stain formulations:
0.1% Ponceau S (w/v) in 5% acetic acid
2% Ponceau S (w/v) in 30% TCA, 30% sulfosalicylic acid

Application

Ponceau S has been used for membrane staining (Western blotting.

Packaging

10, 50, 100 g in glass bottle

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Sex difference in the expression of DNA methyltransferase 3a (DNMT3a) in the rat amygdala during development
MH Kolodkin and AP Auger
Journal of Neuroendocrinology (2011)
Characterization of stress response in human retinal epithelial cells
Journal of Cellular and Molecular Medicine (2012)
Ionizing Radiation?dependent ?-H2AX Focus Formation Requires Ataxia Telangiectasia Mutated and Ataxia Telangiectasia Mutated and Rad3-related
Joanna D. Friesner
Molecular Biology of the Cell (2005)
Bernard L. Horecker, Earl R. Stadtman
Current Topics in Cellular Regulation (2014)
Saif Hossain et al.
mBio, 11(2) (2020-04-23)
Protein homeostasis is critical for proliferation and viability of all organisms. For Candida albicans, protein homeostasis also modulates the transition between yeast and filamentous forms, which is critical for virulence. A key regulator of morphogenesis is the molecular chaperone Hsp90...

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