MilliporeSigma
All Photos(1)

P8074

Sigma-Aldrich

TWEEN® 80

BioXtra, viscous liquid

Synonym(s):
POE (20) sorbitan monooleate, Polyethylene glycol sorbitan monooleate, Polyoxyethylenesorbitan monooleate, Polysorbate 80
CAS Number:
MDL number:
NACRES:
NA.21

description

non-ionic

Quality Level

product line

BioXtra

form

viscous liquid

mol wt

micellar avg mol wt 79,000
average mol wt 1310

composition

Oleic acid, ~70% (balance primarily linoleic, palmitic, and stearic acids)

aggregation number

60

technique(s)

DNA extraction: suitable
RNA extraction: suitable
cell culture | mammalian: suitable
protein purification: suitable

impurities

≤0.025% Phosphorus (P)

ign. residue

<0.1%

CMC

0.012 mM (20-25°C)

transition temp

cloud point 65 °C

density

1.06 g/mL at 25 °C

anion traces

chloride (Cl-): <0.05%
sulfate (SO42-): <0.05%

cation traces

Al: <0.0005%
Ca: <0.001%
Cu: <0.0005%
Fe: <0.0005%
K: <0.005%
Mg: <0.0005%
NH4+: <0.05%
Na: <0.05%
Pb: <0.001%
Zn: <0.0005%

HLB

15

InChI

1S/C32H60O10/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-30(36)40-25-24-37-27-29(39-22-19-34)32-28(38-21-18-33)26-31(42-32)41-23-20-35/h9-10,28-29,31-35H,2-8,11-27H2,1H3/b10-9+

InChI key

RGPBUVUVZKQNHD-MDZDMXLPSA-N

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description

non-ionic

description

non-ionic

description

-

description

non-ionic

product line

BioXtra

product line

-

product line

-

product line

-

form

viscous liquid

form

viscous liquid

form

liquid

form

viscous liquid

mol wt

micellar avg mol wt 79,000, average mol wt 1310

mol wt

average mol wt ~1310

mol wt

-

mol wt

~1228

aggregation number

60

aggregation number

-

aggregation number

-

aggregation number

-

technique(s)

DNA extraction: suitable, RNA extraction: suitable, cell culture | mammalian: suitable, protein purification: suitable

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable

technique(s)

-

technique(s)

DNA extraction: suitable, RNA extraction: suitable, cell culture | mammalian: suitable, protein purification: suitable

General description

["TWEEN"] 80 is a polyethylene sorbitol ester, broadly used in biochemical applications like solubilizing proteins, isolating nuclei from cells in culture, and growing tubercle bacilli. It is also useful in emulsifying and dispersing substances in medicinal and food products. TWEEN 80 acts as an active drying agent in ice cream.

Application

["TWEEN"] 80 has been used:
  • to dissolve rapamycin (RAPA) for immunosuppressive treatment to perform in vivo studies in mice
  • to dissolve remodelin and remodelin Fluor to assess remodelin toxicity in vivo and small molecule dosing
  • to prepare assay medium for the microbiological assay for total folate

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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