P8074

TWEEN^® 80

BioXtra, viscous liquid

• Synonym(s): POE (20) sorbitan monooleate, Polyethylene glycol sorbitan monooleate, Polyoxyethylenesorbitan monooleate, Polysorbate 80
• CAS Number: 9005-65-6
• MDL number: MFCD00082107
• NACRES: NA.21

Properties

• description: non-ionic
• Quality Level: 200
• product line: BioXtra
• form: viscous liquid
• mol wt: micellar avg mol wt 79,000; average mol wt 1310
• composition: Oleic acid, ~70% (balance primarily linoleic, palmitic, and stearic acids)
• aggregation number: 60
• technique(s): DNA extraction: suitable; RNA extraction: suitable; cell culture | mammalian: suitable; protein purification: suitable
• impurities: ≤0.025% Phosphorus (P)
• ign. residue: <0.1%
• CMC: 0.012 mM (20-25°C)
• transition temp: cloud point 65 °C
• density: 1.06 g/mL at 25 °C
• anion traces: chloride (Cl^-): <0.05%; sulfate (SO₄^2-): <0.05%
• cation traces: Al: <0.0005%; Ca: <0.001%; Cu: <0.0005%; Fe: <0.0005%; K: <0.005%; Mg: <0.0005%; NH₄⁺: <0.05%; Na: <0.05%; Pb: <0.001%; Zn: <0.0005%
• HLB: 15
• InChI: 1S/C32H60O10/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-30(36)40-25-24-37-27-29(39-22-19-34)32-28(38-21-18-33)26-31(42-32)41-23-20-35/h9-10,28-29,31-35H,2-8,11-27H2,1H3/b10-9+
• InChI key: RGPBUVUVZKQNHD-MDZDMXLPSA-N

Description

General description

["TWEEN"] 80 is a polyethylene sorbitol ester, broadly used in biochemical applications like solubilizing proteins, isolating nuclei from cells in culture, and growing tubercle bacilli. It is also useful in emulsifying and dispersing substances in medicinal and food products. TWEEN 80 acts as an active drying agent in ice cream.

Application

["TWEEN"] 80 has been used:

• to dissolve rapamycin (RAPA) for immunosuppressive treatment to perform in vivo studies in mice
• to dissolve remodelin and remodelin Fluor to assess remodelin toxicity in vivo and small molecule dosing
• to prepare assay medium for the microbiological assay for total folate

Legal Information

TWEEN is a registered trademark of Croda International PLC

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves