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PPB011

Tris Buffered Saline

powder, pH 7.6, pHast Pack™

Synonym(s):

TBS

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20 pkg

Available to ship TODAYfromMILWAUKEE

$116.00
$87.00

About This Item

UNSPSC Code:
12161700
NACRES:
NA.25
MDL number:
MFCD00132476

$87.00

List Price$116.00Save 25%
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Product Name

Tris Buffered Saline, pH 7.6, pHast Pack™, powder

product line

pHast Pack™

Quality Segment

100

form

powder

storage condition

(Tightly closed. Dry)

impurities

≤5 ppm Heavy Metals (lead)

color

white

pH

7.6

solubility

soluble, clear, colorless

cation traces

Fe: ~10 ppm

foreign activity

DNase, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, Protease, none detected, RNase, none detected

storage temp.

room temp

General description

Tris Buffered Saline, or TBS, pH 7.6 is a commonly used wash buffer that is isotonic and non-toxic and ideal for use in cell culture and electrophoresis applications.

Application

Tris-buffered saline can be used as a wash buffer for:
  • Enzyme Linked Immunosorbent assay (ELISA)
  • Western Blot and other protein applications
  • Suitable for use in cell culture (recommend to sterile filter)
  • Immunohistochemistry
  • Sample preparation
  • Resuspension and sonication of cells

Features and Benefits

  • Ready for use, just dissolve in ultrapure water
  • No calculations, measuring, and pH adjustments needed
  • Save time and minimize effort
  • Biological tests: free of DNase, RNase, Protease, and Nickase
  • Chemical tests: Iron ≤10 ppm, Lead ≤5 ppm

Packaging

Foil pouches

Preparation Note

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 1x solution containing 50 mM Tris, 138 mM NaCl, 2.7 mM KCl, pH 7.6 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free. This product is suitable for use in cell culture applications.
Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

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Quality Level

100

Quality Level

-

Quality Level

200

Quality Level

200

product line

pHast Pack™

product line

pHast Pack™

product line

-

product line

-

form

powder

form

powder

form

powder

form

solution

storage condition

(Tightly closed. Dry)

storage condition

(Tightly closed. Dry)

storage condition

-

storage condition

-

storage temp.

room temp

storage temp.

room temp

storage temp.

-

storage temp.

2-8°C

pH

7.6

pH

8.0

pH

8.0

pH

7.2—7.6

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Storage Class

11 - Combustible Solids

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