R1148

Supelco

Rogosa SL agar

suitable for microbiology, NutriSelect® Plus

NACRES:
NA.85

Quality Level

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

manufacturer/tradename

NutriSelect® Plus

application(s)

bioburden testing: suitable
microbiological culture: suitable
microbiology: suitable

final pH

5.4±0.2 (25 °C)

Featured Industry

Clinical Testing
Food and Beverages

storage temp.

2-8°C

suitability

selective by low pH for Lactobacillus spp.

Related Categories

Application

Used as a selective medium for cultivation of Lactobacilli.

Components

Ingredients (g/L)
Tryptose, 10.00
Yeast extract, 5.00
Dextrose, 10.00
Arabinose, 5.00
Saccharose, 5.00
Sodium acetate, 15.00
Ammonium citrate, 2.00
Monopotassium phoshate, 6.00
Magnesium sulphate, 0.57
Manganese sulphate, 0.12
Ferrous sulphate, 0.03
Polysorbate 80, 1.00
Agar, 15.00

Preparation Note

Suspend 75 g of Rogosa SL Agar in 1000 ml of distilled water. Boil to dissolve the medium completely. Add 1.32 ml of glacial acetic acid (Cat. No. 45726). Mix thoroughly, and distribute into culture tubes or flasks. Heat to 90-100°C for 2-3 minutes. Cool to 45°C for direct inoculation.
DO NOT AUTOCLAVE.

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
A selective medium for the isolation and enumeration of oral and fecal lactobacilli.
M ROGOSA et al.
Journal of bacteriology, 62(1), 132-133 (1951-07-01)
Articles
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