SBR00025

Novobiocin Ready Made Solution

100 mg/mL in water

• Synonym(s): Novobiocin Ready Made Solution
• Linear Formula: C₃₁H₃₅N₂NaO₁₁
• Molecular Weight: 634.61
• UNSPSC Code: 51101500
• NACRES: NA.76

Properties

• biological source: microbial
• assay: ≥90%
• form: liquid
• concentration: 100 mg/mL in water
• color: faint yellow to dark brown
• antibiotic activity spectrum: Gram-negative bacteria; Gram-positive bacteria; mycobacteria; neoplastics
• mode of action: DNA synthesis | interferes; enzyme | inhibits
• storage temp.: −20°C

Description

General description

Novobiocin, also known as albamycin or cathomycin, is an aminocoumarin antibiotic commonly derived from Streptomyces Sp. It exerts its antibacterial activity by inhibiting Topo II α, which corresponds to bacterial DNA gyrase. It is effective against both gram-positive and gram-negative bacteria and is significant in research involving highly beta-lactam-resistant pneumococcal infections and anti-orthopoxvirus applications. Functioning as a competitive inhibitor in the ATPase reaction catalyzed by GyrB, Novobiocin is employed in microbiology and cell biology research to effectively prevent the growth of bacterial contaminants.

Application

Novobiocin has been used:

• in cell culture systems to treat mycoplasma contaminations.[3]
• in a research to where it has exhibited anti-cancer effects by inhibition of Hsp90 (heat shock protein)[4],[5]

Biochem/physiol Actions

Mode of Action: Inhibits DNA synthesis by inhibiting the enzyme Topoisomerase II

Activity spectrum: Active against gram-positive and gram-negative bacteria

Features and Benefits

• Ready available solution reduce the need for preparation time
• Commonly used in Cell Biology and Biochemical applications
• High quality antibiotic suitable for mulitple research applications

Preparation Note

Novobiocin Ready Made Solution is light sensitive. Thaw this product at room temperature. It is recommended to avoid repeated freeze thaw cycles of this product. The suggested concentration of Novobiocin for cell culture assays is 50-1000 μg/mL,^,, therefore, it is recommended to dilute the Ready Made Solution 1:100-2000.

Other Notes

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Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• wgk_germany: WGK 1