Sodium thiosulfate is used to remove any unreacted silver to prevent increased background staining, per the Instructions For Use. These instructions also specify the suitability of well-prepared tissue sections cut 5–6 microns and fixed in neutral buffered formalin for iron staining, as well as the preparation method for blood or bone marrow films.
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form
liquid
Quality Level
usage
sufficient for ≥100 slides staining and fixing
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-8°C
Application
Features and Benefits
Packaging
Principle
Kit Components Only
- Sodium thiosulfate pentahydrate, reagent grade, ≥99.5% 500 g
signalword
Danger
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Repr. 1B
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
flash_point_f
Not applicable
flash_point_c
Not applicable
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What is the fixer Sodium Thiosulfate in Ag enhancer kit SE100 used for?
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For the Silver Enhancer Kit (Cat # SE100), what is the capping agent on the resulting silver nanoparticle that is formed after the silver enhancement reaction?
1 answer-
Am not sure what is meant by the capping reagent. The Silver Enhancer kit shares similarities with the Grocott Methanamine Silver Nitrate (GMS) procedure for fungus, basement membranes or urates. The GMS and SE100 kit are both silver stain methods. The SE100 has been formulated to stain gold nanoparticles instead of fungi, basement membranes, or urates. The SE100 has 3 reagents. Reagent 1 is a silver nitrate solution. Solution 2 is a Tungstosilicic Acid Hydrate solution. This compound likely replaces the methenamine reagent typically used in the GMS procedure. Gold Chloride is considered a toning solution in the GMS procedure. There is not an equivalent reagent in the SE100 procedure. The third reagent in the SE100 kit is a sodium thiosulfate solution which removes any unreduced silver. It is likely that the Tungstosilicic Acid Hydrate is what you would refer to as a capping agent.
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