MilliporeSigma
All Photos(1)

T15760

Sigma-Aldrich

Trizma® hydrochloride

reagent grade, ≥99%, crystalline

Sign Into View Organizational & Contract Pricing

Select a Size

Synonym(s):
TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride
Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
Molecular Weight:
157.60
Beilstein:
3675235
MDL number:

biological source

synthetic

Quality Level

grade

reagent grade

Assay

≥99%

form

crystalline

useful pH range

7.0-9.0

pKa (25 °C)

8.1

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
T3253T6666T5941
Trizma® hydrochloride reagent grade, ≥99%, crystalline

Sigma-Aldrich

T15760

Trizma® hydrochloride

Essential Grade
Trizma® hydrochloride reagent grade, ≥99.0% (titration), crystalline

Sigma-Aldrich

T3253

Trizma® hydrochloride

Essential Grade
Trizma® hydrochloride pH 3.5-5.0 (0.5 M in H2O), BioXtra, ≥99.0% (titration)

Sigma-Aldrich

T6666

Trizma® hydrochloride

Premium Grade
Trizma® hydrochloride BioPerformance Certified, suitable for cell culture, ≥99.0% (titration)

Sigma-Aldrich

T5941

Trizma® hydrochloride

Premium Grade
assay

≥99%

assay

≥99.0% (titration)

assay

≥99.0% (titration)

assay

≥99.0% (titration)

form

crystalline

form

crystalline

form

powder

form

crystalline powder

Quality Level

200

Quality Level

400

Quality Level

400

Quality Level

400

biological source

synthetic

biological source

-

biological source

-

biological source

-

useful pH range

7.0-9.0

useful pH range

7.0-9.0

useful pH range

7.0-9.0

useful pH range

7.0-9.0

General description

Tris HCl, or Trizma® hydrochloride, serves as a widely utilized acidic buffer with a pKa of 8.08 at 25°C, offering a pH range of 7.0 to 9.0, aligning with the typical pH of living organisms. It is a crucial component in buffer solutions for diverse biological, cell biology, and biochemistry applications. Notably, Tris base and Tris hydrochloride individually lack sufficient buffering capacity, necessitating their combination to create effective buffers within the pH range of 7 to 9.

Essential in protein electrophoresis and western blotting techniques, Tris buffers, particularly low ionic strength versions, are employed for protein transfer. They are also commonly used in SDS-PAGE gels, running buffers, and blotting buffers. Additionally, Tris salts, integral to DNA agarose electrophoresis, contribute to buffer preparations like TAE (Tris acetate/EDTA) and TBE (Tris borate/EDTA), playing pivotal roles in biochemistry, protein purification, and electrophoresis applications. Overall, Tris HCl proves to be a versatile and indispensable component, ensuring optimal pH in biological systems for effective biochemical and cell biology research.

Features and Benefits

  • Ideal as a Biological buffer for Cell Biology and Biochemical research
  • Effective Buffering from pH 7-9 (25 °C) with a pKa of 8.1 (25 °C)
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications

Other Notes

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
The pH values of all buffers are temperature and concentration-dependent. For Tris buffers, pH increases by about 0.03 unit per °C decrease in temperature and decreases by 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service