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T3260

Sigma-Aldrich

Toluidine Blue O

Technical grade

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Synonym(s):
Basic Blue 17, Blutene chloride, Methylene Blue T50 or T extra, Tolonium chloride
Empirical Formula (Hill Notation):
C15H16ClN3S
CAS Number:
Molecular Weight:
305.83
Colour Index Number:
52040
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

form

powder

composition

Dye content, ~80%

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C

InChI

1S/C15H15N3S.ClH/c1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;/h4-8,16H,1-3H3;1H

InChI key

GEDVVYWLPUPJJZ-UHFFFAOYSA-N

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This Item
198161896401.04172
Toluidine Blue O Technical grade

Sigma-Aldrich

T3260

Toluidine Blue O

Toluidine Blue O certified by the Biological Stain Commission

Sigma-Aldrich

198161

Toluidine Blue O

Toluidine Blue for microscopy (Hist., Vit.)

Sigma-Aldrich

89640

Toluidine Blue

composition

Dye content, ~80%

composition

Dye content, 80%

composition

-

composition

Dye, ≥85% spectrophotometric assay

solubility

H2O: 1 mg/mL

solubility

-

solubility

methanol: 0.01 g/10 mL, blue to very deep blue

solubility

30 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

2-30°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Toluidine Blue O (TBO) is a thiazine dye of the quinone-imine family and is cationic in nature. It has been employed in polychromatic staining of paraffin embedded plant cell walls. It is a metachromatic dye suitable for a wide variety of histological staining procedures.
A metachromatic dye suitable for a wide variety of histological staining procedures.

Application

Toluidine Blue O has been used to stain a variety of specimens like mesenchymal cells, taste buds, osteoblasts, matrix, etc.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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