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T5428

Sigma-Aldrich

Tetramethylrhodamine methyl ester perchlorate

≥95%

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Synonym(s):
TMRM
Linear Formula:
C25H25N2O3 · ClO4
CAS Number:
Molecular Weight:
500.93
Beilstein:
7162165
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

Assay

≥95%

form

powder

solubility

methanol: 5 mg/mL, clear

εmax

>95000 at 546-552 nm in methanol

fluorescence

λem 573 nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[O-]Cl(=O)(=O)=O.COC(=O)c1ccccc1C2=C3C=C\C(C=C3Oc4cc(ccc24)N(C)C)=[N+](\C)C

InChI

1S/C25H25N2O3.ClHO4/c1-26(2)16-10-12-20-22(14-16)30-23-15-17(27(3)4)11-13-21(23)24(20)18-8-6-7-9-19(18)25(28)29-5;2-1(3,4)5/h6-15H,1-5H3;(H,2,3,4,5)/q+1;/p-1

InChI key

PFYWPQMAWCYNGW-UHFFFAOYSA-M

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1 of 4

This Item
88997T3163T0820
form

powder

form

powder

form

powder

form

powder

solubility

methanol: 5 mg/mL, clear

solubility

DMF: 2 mg/mL, clear, acetonitrile: soluble

solubility

-

solubility

-

εmax

>95000 at 546-552 nm in methanol

εmax

-

εmax

-

εmax

82-98 at 540-546 nm in methanol

fluorescence

λem 573 nm in methanol

fluorescence

λex 543 nm; λem 575 nm in 0.1 M phosphate pH 7.0

fluorescence

-

fluorescence

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

General description

Tetramethylrhodamine methyl ester perchlorate (TMRM) is a membrane-permeant, cationic, fluorescent dye that can label mitochondria and mark embryonic stem cell/induced pluripotent stem cell-derived cardiomyocytes. It is useful for the isolation of mature cardiomyocytes.

Application

Tetramethylrhodamine methyl ester perchlorate (TMRM) has been used as a sensitive probe for the detection of mitochondrial membrane potential.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Emerging Trends in Cell and Gene Therapy null
Therapeutic Targeting of the Mitochondria Initiates Excessive Superoxide Production and Mitochondrial Depolarization Causing Decreased mtDNA Integrity
Kaytee L
PLoS ONE (2016)
Yuejiao Ma et al.
Journal of cell science, 132(15) (2019-07-11)
Mitochondrial remodelling is a central feature of stem cell differentiation. However, little is known about the regulatory mechanisms during these processes. Previously, we found that a pharmacological inhibitor of glycogen synthase kinase-3α and -3β, CHIR-99021, initiates human adipose stem cell
Quantitative analysis of mitochondrial morphology and membrane potential in living cells using high-content imaging, machine learning, and morphological binning
Anthony P
Biochimica et Biophysica Acta (2015)
Antigoni Diokmetzidou et al.
Journal of cell science, 129(20), 3705-3720 (2016-08-28)
The association of desmin with the α-crystallin Β-chain (αΒ-crystallin; encoded by CRYAB), and the fact that mutations in either one of them leads to heart failure in humans and mice, suggests a potential compensatory interplay between the two in cardioprotection.

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