T5428

Tetramethylrhodamine methyl ester perchlorate

Name: Tetramethylrhodamine methyl ester perchlorate
Brand: SIAL
Price: 308 USD
Availability: InStock

≥95% purity, powder

Synonym(s):

TMRM

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25 MG

$308.00

Estimated to ship onFebruary 16, 2026Details

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About This Item

Linear Formula:

C₂₅H₂₅N₂O₃ · ClO₄

CAS Number:

115532-50-8

Molecular Weight:

500.93

NACRES:

NA.47

PubChem Substance ID:

24900280

UNSPSC Code:

12171500

MDL number:

MFCD00274460

Beilstein/REAXYS Number:

7162165

Key Documents

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Product Name

Tetramethylrhodamine methyl ester perchlorate, ≥95%

InChI

1S/C25H25N2O3.ClHO4/c1-26(2)16-10-12-20-22(14-16)30-23-15-17(27(3)4)11-13-21(23)24(20)18-8-6-7-9-19(18)25(28)29-5;2-1(3,4)5/h6-15H,1-5H3;(H,2,3,4,5)/q+1;/p-1

SMILES string

[O-]Cl(=O)(=O)=O.COC(=O)c1ccccc1C2=C3C=C\C(C=C3Oc4cc(ccc24)N(C)C)=[N+](\C)C

InChI key

PFYWPQMAWCYNGW-UHFFFAOYSA-M

assay

≥95%

form

powder

composition

Carbon: 57.5-62.4%

Nitrogen: 5.3-5.9%

technique(s)

titration: suitable

color

dark green

solubility

methanol: 5 mg/mL, clear

ε_max

>95000 at 546-552 nm in methanol

fluorescence

λ_em 573 nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

Quality Level

200

Related Categories

Clinical & Diagnostics

Hematology Stains

Histology Stains

Tissue Diagnostics

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Show Differences

1 of 4

This Item	88997	T3163	94506
Sigma-Aldrich T5428 Tetramethylrhodamine methyl ester perchlorate	Sigma-Aldrich 88997 6-Carboxy-tetramethylrhodamine N-succinimidyl ester	Sigma-Aldrich T3163 Tetramethylrhodamine isothiocyanate Isomer R	Sigma-Aldrich 94506 Tetramethylrhodamine-5-maleimide
technique(s) titration: suitable	technique(s) -	technique(s) -	technique(s) -
fluorescence λ_em 573 nm in methanol	fluorescence λ_ex 543 nm; λ_em 575 nm in 0.1 M phosphate pH 7.0	fluorescence -	fluorescence λ_ex 543 nm; λ_em 575 nm in 0.1 M phosphate pH 7.0
color dark green	color -	color -	color -
form powder	form powder	form powder	form powder
application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology	application(s) -
solubility methanol: 5 mg/mL, clear	solubility DMF: 2 mg/mL, clear, acetonitrile: soluble	solubility 1 M NH4OH: 10 mg/mL (Faint Red to Very Dark Red to Very Dark Purple solution)	solubility DMSO: soluble, methanol: soluble

General description

Tetramethylrhodamine methyl ester perchlorate (TMRM) is a membrane-permeant, cationic, fluorescent dye that can label mitochondria and mark embryonic stem cell/induced pluripotent stem cell-derived cardiomyocytes. It is useful for the isolation of mature cardiomyocytes.[1]

Application

Tetramethylrhodamine methyl ester perchlorate (TMRM) has been used as a sensitive probe for the detection of mitochondrial membrane potential.[2][3]

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Lysine acetylome profiling uncovers novel histone deacetylase substrate proteins in

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Molecular systems biology, 13(10), 949-949 (2017-10-25)

Histone deacetylases have central functions in regulating stress defenses and development in plants. However, the knowledge about the deacetylase functions is largely limited to histones, although these enzymes were found in diverse subcellular compartments. In this study, we determined the

Therapeutic Targeting of the Mitochondria Initiates Excessive Superoxide Production and Mitochondrial Depolarization Causing Decreased mtDNA Integrity

Kaytee L

PLoS ONE (2016)

Desmin and αB-crystallin interplay in the maintenance of mitochondrial homeostasis and cardiomyocyte survival.

Antigoni Diokmetzidou et al.

Journal of cell science, 129(20), 3705-3720 (2016-08-28)

The association of desmin with the α-crystallin Β-chain (αΒ-crystallin; encoded by CRYAB), and the fact that mutations in either one of them leads to heart failure in humans and mice, suggests a potential compensatory interplay between the two in cardioprotection.

CHIR-99021 regulates mitochondrial remodelling via β-catenin signalling and miRNA expression during endodermal differentiation.

Yuejiao Ma et al.

Journal of cell science, 132(15) (2019-07-11)

Mitochondrial remodelling is a central feature of stem cell differentiation. However, little is known about the regulatory mechanisms during these processes. Previously, we found that a pharmacological inhibitor of glycogen synthase kinase-3α and -3β, CHIR-99021, initiates human adipose stem cell

Quantitative analysis of mitochondrial morphology and membrane potential in living cells using high-content imaging, machine learning, and morphological binning

Anthony P

Biochimica et Biophysica Acta (2015)

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