MilliporeSigma
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T8533

Sigma-Aldrich

o-Tolidine

≥95%

Synonym(s):
3,3′-Dimethylbenzidine, 4,4′-Bianisidine
Linear Formula:
[-C6H3(CH3)-4-NH2]2
CAS Number:
Molecular Weight:
212.29
Beilstein:
2210640
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

assay

≥95%

form

powder or chunks

mp

128-131 °C (lit.)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cc1cc(ccc1N)-c2ccc(N)c(C)c2

InChI

1S/C14H16N2/c1-9-7-11(3-5-13(9)15)12-4-6-14(16)10(2)8-12/h3-8H,15-16H2,1-2H3

InChI key

NUIURNJTPRWVAP-UHFFFAOYSA-N

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General description

o-Tolidine is a diacidic dye which is sparingly soluble in water and easily soluble in ether and alcohol. On exposure to chlorine or bromine vapors, it forms an intense blue color. This forms the basis for detection of chlorine, nitrates and hydrogen peroxide in air or water. It turns yellow on reacting with free chlorine. o-Tolidine easily forms acid salts.

Application

o-Tolidine has also been used to determine the presence of laccase activity in T. versicolor fungal colonies.
Sensitive colorimetric reagent for gold and for free chlorine in water.

Packaging

25, 100 g in glass bottle

Warning

May be carcinogenic.

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1B

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

212.0 °F - closed cup

Flash Point(C)

100 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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