T9032

Thioglycollate medium

NutriSelect^® Plus, USP, EP, AOAC^®, powder, pkg of 500 g, pkg of 1000 g, for bacteria

• Synonym(s): FTG, Fluid Thioglycolate, Fluid Thioglycollate Medium, Thioglycollate Broth
• UNSPSC Code: 41171607
• NACRES: NA.85

Properties

• agency: AOAC^®; EP; USP
• Quality Level: 200
• description: Microbiologically tested
• sterility: non-sterile
• form: powder
• packaging: pkg of 1000 g; pkg of 500 g
• manufacturer/tradename: NutriSelect^® Plus
• application(s): clinical testing; environmental; food and beverages; sterility testing
• suitability: bacteria

Description

General description

Thioglycollate Medium is a powdered broth medium designed for the cultivation of both aerobic and anaerobic microorganisms. The medium contains dextrose, tryptone, yeast extract, and L-cystine providing essential growth factors for bacterial proliferation. Sodium thioglycollate and L-cystine serve as reducing agents that prevent the accumulation of lethal peroxides, enabling the growth of Clostridium even under aerobic conditions. Resazurin acts as an oxidation-reduction indicator, appearing pink when oxidized and colorless when reduced. The minimal amount of agar in the medium supports the growth of both aerobes and anaerobes, regardless of the presence of sodium thioglycollate. Sodium thioglycollate also neutralizes the toxic effects of mercurial preservatives and peroxides, promoting anaerobiosis and making the medium suitable for testing materials containing heavy metals. Any increase in oxygen content is indicated by a color change in resazurin from colorless to pink. The small agar content helps maintain a low redox potential, stabilizing the medium.

Application

Thioglycollate Medium is widely used in clinical testing, environmental monitoring, food and beverage testing, and sterility testing. It supports the growth of both aerobic and anaerobic microorganisms, making it ideal for sterility control in pharmaceutical products and for testing materials containing heavy metals with bacteriostatic effects. The medium is also used to determine an organism′s oxygen requirements and its ability to grow in anaerobic or microaerophilic environments.

Features and Benefits

NutriSelect^® Plus is a powdered culture media offering

• Quality control with growth promotion test according to industry standards
• Quality assurance acc. to ISO 9001
• Cost-efficient powdered media

Preparation Note

Solve 29.8 g in 1 litre distilled water. Autoclave 15 minutes at 121°C.

Other Notes

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

AOAC is a registered trademark of AOAC International

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable