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T9284

Sigma-Aldrich

Triton X-100

BioXtra

Synonym(s):
t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
Linear Formula:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS Number:
Beilstein:
2315025
MDL number:
PubChem Substance ID:
NACRES:
NA.21

description

non-ionic

Quality Level

product line

BioXtra

form

liquid

mol wt

micellar avg mol wt 80,000
average mol wt 625

aggregation number

100-155

technique(s)

permeability assay: suitable

impurities

≤0.05% Phosphorus (P)

ign. residue

≤0.4%

CMC

0.2-0.9 mM (20-25°C)

transition temp

cloud point 65 °C
pour point ~7 °C

solubility

water: soluble

anion traces

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%

cation traces

Al: ≤0.0005%
Ca: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.05%
Na: ≤0.1%
Pb: ≤0.001%
Zn: ≤0.0005%

HLB

13.5

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

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This Item
648463X114648466
Triton™ X-100 BioXtra

Sigma-Aldrich

T9284

Triton X-100

Triton™ X-114 laboratory grade

Sigma-Aldrich

X114

Triton X-114

product line

BioXtra

product line

-

product line

-

product line

-

form

liquid

form

liquid

form

-

form

liquid

mol wt

micellar avg mol wt 80,000, average mol wt 625

mol wt

micellar avg mol wt 80,000, average mol wt 625

mol wt

average mol wt 537

mol wt

micellar avg mol wt 80,000, average mol wt 625

aggregation number

100-155

aggregation number

100-155

aggregation number

-

aggregation number

100-155

technique(s)

permeability assay: suitable

technique(s)

-

technique(s)

-

technique(s)

-

General description

Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Application

Triton X-100 has been used:
  • Along with Span® 80 and Tween® 80 in mineral oil for the preparation of oil-phase of microemulsions for PCR technique
  • As a component of NX buffer which is used to pool and break down the microemulsions after PCR cycling
  • In the blocking solution during immunocytochemistry procedure
  • During deglycosylation procedure
  • For membrane solubilization
  • For cell permeabilization
  • In decellularization of porcine heart valve tissue

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Legal Information

Span is a registered trademark of Croda International PLC
TWEEN is a registered trademark of Croda International PLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

also commonly purchased with this product

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

483.8 °F

Flash Point(C)

251 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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