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T9284

Sigma-Aldrich

Triton X-100

BioXtra

Synonym(s):
t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
Linear Formula:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS Number:
Beilstein:
2315025
MDL number:
PubChem Substance ID:
NACRES:
NA.21

description

non-ionic

Quality Level

product line

BioXtra

form

liquid

mol wt

micellar avg mol wt 80,000
average mol wt 625

aggregation number

100-155

technique(s)

permeability assay: suitable

impurities

≤0.05% Phosphorus (P)

ign. residue

≤0.4%

CMC

0.2-0.9 mM (20-25°C)

transition temp

cloud point 65 °C
pour point ~7 °C

solubility

water: soluble

anion traces

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%

cation traces

Al: ≤0.0005%
Ca: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.05%
Na: ≤0.1%
Pb: ≤0.001%
Zn: ≤0.0005%

HLB

13.5

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

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General description

Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Application

Triton X-100 has been used:
  • Along with Span® 80 and Tween® 80 in mineral oil for the preparation of oil-phase of microemulsions for PCR technique
  • As a component of NX buffer which is used to pool and break down the microemulsions after PCR cycling
  • In the blocking solution during immunocytochemistry procedure
  • During deglycosylation procedure
  • For membrane solubilization
  • For cell permeabilization
  • In decellularization of porcine heart valve tissue

Packaging

100, 500 mL in poly bottle
1 L in poly bottle

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Legal Information

Span is a registered trademark of Croda International PLC
TWEEN is a registered trademark of Croda International PLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

483.8 °F

Flash Point(C)

251 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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