Wilkins Chalgren anaerobic agar

suitable for microbiology, NutriSelect® Plus


Quality Level





shelf life

limited shelf life, expiry date on the label


NutriSelect® Plus


microbiological culture: suitable
microbiology: suitable

final pH

7.1±0.2 (25 °C)

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Cleaning Products
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anaerobic bacteria
selective for Bacteroides spp.
selective for Bifidobacterium spp.
selective for Clostridium spp.


Used for the isolation of anaerobic bacteria.


Ingredients (g/L)
Casein enzymatic hydrolysate, 10.00
Peptic digest of animal tissue, 10.00
Yeast extract, 5.00
Dextrose, 1.00
Sodium chloride, 5.00
L-Arginine, 1.00
Sodium pyruvate, 1.00
Hemin, 0.005
Menadione, 0.0005
Agar, 10.00

Preparation Note

Suspend 43 g of Wilkins Chalgren Anaerobic Agar in 1000 ml of distilled water. Heat to boiling to dissolve the medium completely. Dispense and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 50°C before adding antibiotics to be tested. Mix gently and pour into sterile petri plates.For the cultivation of anaerobes add 2 vial of Non-Spore Anaerobic Supplement (Cat. No. N8781) or 2 vial of G.N. Spore Anaerobic Supplement (Cat. No. G3789) as desired into the sterile molten medium before pouring into sterile petri plates.

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany


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Hazard Statements

Precautionary Statements

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
T D Wilkins et al.
Antimicrobial agents and chemotherapy, 10(6), 926-928 (1976-12-01)
A medium is described which was designed for use in testing the minimal inhibitory concentration of antibiotics for anaerobic bacteria by agar dilution. It contains: Trypticase (1%), Gelysate (1%), yeast extract (0.5%), glucose (0.1%), pyruvate (0.1%), arginine (0.1%), NaCl (0.5%)...
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