Heating this product to 55°C may lead to changes in viscosity, solubility, or performance characteristics. There is no indication that this product can attack aluminum and titanium metal layers. This product is generally compatible with diluted nitric acid. While no specific incompatibility is reported, the combination may alter the properties of both the surfactant and the acid, potentially affecting the performance of either in applications such as cleaning or etching. Additionally, it is important to consider the concentration and the potential for foaming or other reactions during mixing.
X100
Triton™ X-100
Triton™ X-100
laboratory grade
Synonym(s):
t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
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5 ML
$17.10
100 ML
$57.90
500 ML
$73.30
1 L
$106.00
1 GAL
$252.00
5 GAL
$842.00
$17.10
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Available to ship onMay 13, 2025Details
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5 ML
$17.10
100 ML
$57.90
500 ML
$73.30
1 L
$106.00
1 GAL
$252.00
5 GAL
$842.00
About This Item
Linear Formula:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS Number:
Beilstein/REAXYS Number:
2315025
MDL number:
UNSPSC Code:
12161900
PubChem Substance ID:
NACRES:
NA.21
$17.10
List Price$19.00Save 10%Web-Only Promotion
Available to ship onMay 13, 2025Details
Request a Bulk Order
Recommended Products
biological source
synthetic
Quality Level
grade
laboratory grade
description
non-ionic
form
liquid
mol wt
micellar avg mol wt 80,000
average mol wt 625
aggregation number
100-155
CMC
0.2-0.9 mM (20-25°C)
mp
6 °C (43 °F)
transition temp
cloud point 65 °C
pour point ~7 °C
solubility
water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow
HLB
13.5
SMILES string
CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
InChI
1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
InChI key
HNLXNOZHXNSSPN-UHFFFAOYSA-N
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General description
Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.[3][4]
Application
Biochem/physiol Actions
Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.
Features and Benefits
- Ideal for Cell Biology and Biochemical research applications
- Non-ionic nondenaturing detergent
- Superior wetting agent and emulsifier
- Highly versatile surfactant
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Legal Information
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
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Description
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signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
483.8 °F - closed cup
flash_point_c
251 °C - closed cup
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter, type N95 (US)
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Articles
Millicell® hanging cell culture inserts for staining and microscopic analysis directly from the insert. Learn to utilize hanging cell culture inserts in barrier measurements.
Protocols
Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.
Enzymatic activity assay for cholesterol oxidase, excluding specific product numbers, providing guidelines for accurate cholesterol oxidase assays.
This page shows how to solubilize membrane proteins with products from Cytiva.
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What kind of change will be observed if heat up to 55C? Will it attack stacked Al and Ti metal layer? Can it mix with diluted nitric acid to etch Cu/NiV?
1 answer-
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Is this product nuclease-free?
1 answer-
The product is not tested for RNase or DNase. Please see the link below to review Triton X-100 product options that include RNase and DNase testing:
https://www.sigmaaldrich.com/product/compare?term=triton%20x-100%20nuclease&focus=products&page=1&perpage=30&sort=relevance&type=product&products=T8787%3ASIGMA%3Asial%2C648466%3AMM%3Asial&returnUrl=%2Fsearch%2Ftriton-x-100-nucleaseHelpful?
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How long is Triton X-100 stable at -80 degrees in buffer?
1 answer-
At -80°C, Triton X-100 is generally stable, but the stability can depend on the specific buffer conditions and the concentration of the detergent. In most cases, Triton X-100 remains effective at low temperatures, but it is advisable to avoid repeated freeze-thaw cycles, as these can lead to changes in the detergent's properties and effectiveness. Please note that the shelf life after opening is dependent upon various factors which might affect the stability and, therefore, it is not guaranteed and needs to be determined by the end user.
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If a sterilization of Triton X-100 or its dilutions (maximum 10% of Triton X-100 in the total volume) is needed to remove DNAse and RNAse, what's the best approach?
1 answer-
Autoclaving undiluted Triton X-100 is generally not recommended for sterilization. However, dilute solutions, up to 10%, may be autoclaved. At certain
concentrations, solutions may become cloudy, but dispersible above the cloud point. The solution will clarify with stirring upon cooling. Note that DNAse and RNAse-free products are currently available. Please see the links below to review these product options:
https://www.sigmaaldrich.com/product/sigma/t8787
https://www.sigmaaldrich.com/product/mm/648466
https://www.sigmaaldrich.com/product/sigma/93443Helpful?
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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What are the storage conditions for the Triton 100x? It's not in the COA
1 answer-
This product may be be stored in room temperature.
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Will heating Triton to reduce its viscosity have any negative impact on the product? Does heating negatively affect the activity of Triton?
1 answer-
Triton X-100 can be frozen, thawed, and used. However, caution is advised when heating it above 50°C, as discoloration may occur. This note indicates that excessive heating can lead to some form of change in Triton X-100. It would be reasonable to assume that the recommended working range is from ambient temperature to 50°C (or slightly lower). If your process requires heating the solution to temperatures higher than 50°C, it is recommended to add Triton X-100 only after the temperature has dropped below 50°C or lower. If you need to work at temperatures exceeding 50°C, it is advisable to conduct solution stability studies to assess potential damage or breakdown of Triton X-100.
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Is a concentration of 8x10^-5 mol/L of Triton X-100 harmful to metal (steel/aluminum) and silicon equipment when submerged in water?
1 answer-
The laboratory has not specifically tested the corrosive properties of Triton X-100 on metal. However, according to a publication titled "Triton X-100 and Sodium Lauryl Sulfate Surfactants as Environmentally Friendly Corrosion Inhibitors for Steel in 0.5 M H2SO4" in Advances in Analytical Chemistry, Triton X-100 appears to act as a corrosion inhibitor for steel.
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What is the correct CAS number for Triton X-100, 9002-93-1 or 9036-19-5?
1 answer-
CAS 9036-19-5 is a generic CAS number and is correct for Triton® X-100. With this number, neither the substitution pattern of the aromatic ring (para, meta, ortho), nor the amount of the repeating units of the oxyethylene units is specified or described. Triton® X-100 with CAS 9002-93-1 is also correct but it defines only the para-substitution.
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