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X100PC

Sigma-Aldrich

Triton X-100

peroxide- and carbonyl-free

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Synonym(s):
t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
Linear Formula:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS Number:
Beilstein/REAXYS Number:
2315025
MDL number:
PubChem Substance ID:

description

non-ionic

Quality Level

form

liquid

mol wt

micellar avg mol wt 80,000
average mol wt 625

packaging

ampule of 5, 5x5, 10x5 mL

aggregation number

100-155

CMC

0.2-0.9 mM (20-25°C)

transition temp

cloud point 65 °C
pour point ~7 °C

solubility

water: soluble

HLB

13.5

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

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1 of 4

This Item
T9284X100X114
Triton™ X-100 peroxide- and carbonyl-free

X100PC

Triton X-100

-
Triton™ X-100 BioXtra

T9284

Triton X-100

Premium Grade
Triton™ X-100 laboratory grade

X100

Triton X-100

-
Triton™ X-114 laboratory grade

X114

Triton X-114

-
aggregation number

100-155

aggregation number

100-155

aggregation number

100-155

aggregation number

-

transition temp

cloud point 65 °C, pour point ~7 °C

transition temp

cloud point 65 °C, pour point ~7 °C

transition temp

cloud point 65 °C, pour point ~7 °C

transition temp

pour point ~−9 °C, cloud point 23 °C

CMC

0.2-0.9 mM (20-25°C)

CMC

0.2-0.9 mM (20-25°C)

CMC

0.2-0.9 mM (20-25°C)

CMC

0.2 mM (20-25°C)

HLB

13.5

HLB

13.5

HLB

13.5

HLB

12.4

mol wt

micellar avg mol wt 80,000, average mol wt 625

mol wt

micellar avg mol wt 80,000, average mol wt 625

mol wt

micellar avg mol wt 80,000, average mol wt 625

mol wt

average mol wt 537

General description

Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Application

0·3% Triton X-100 in TBS has been used for permeabilization of retinas before immunostaining. 0.5% Triton X-100 has been used for astrocyte cell lysis.

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Packaging

Packaged in ampules under Argon.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

483.8 °F

flash_point_c

251 °C

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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Triton™ X-100 Detergent Non-ionic detergent and emulsifier. Aggregation number: 100-155.

Millipore

648462

Triton X-100 Detergent

Adeno-associated virus mediated SOD gene therapy protects the retinal ganglion cells from chronic intraocular pressure elevation induced injury via attenuating oxidative stress and improving mitochondrial dysfunction in a rat model.
American Journal of Translational Research, 8(2), 799-799 (2016)
Quantitative ex vivo detection of rodent retinal ganglion cells by immunolabeling Brn-3b.
Experimental Eye Research, 79(1), 131-140 (2004)
Cytotoxicity of bilirubin for human fibroblasts and rat astrocytes in culture. Effect of the ratio of bilirubin to serum albumin.
Chuniaud L, et al.
Clinica Chimica Acta; International Journal of Clinical Chemistry, 256(2), 103-114 (1996)
The size, shape, and hydration of nonionic surfactant micelles. Triton X-100.
Robson RJ and Dennis EA
The Journal of Physical Chemistry, 81, 1075-1078 (1977)
Triton X-100 concentration effects on membrane permeability of a single HeLa cell by scanning electrochemical microscopy (SECM).
Koley D and Bard AJ
Proceedings of the National Academy of Sciences of the USA, 107, 16783-16783 (2010)

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