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MilliporeSigma

X100PC

Triton™ X-100

peroxide- and carbonyl-free

Synonym(s):

t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether

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5 ML

$194.00

5 X 5 ML

$647.00

10 X 5 ML

$1,110.00

$194.00

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About This Item

Linear Formula:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS Number:
9036-19-5
UNSPSC Code:
12161900
NACRES:
NA.25
PubChem Substance ID:
329830773
MDL number:
MFCD00128254
Beilstein/REAXYS Number:
2315025

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InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

description

non-ionic

form

liquid

mol wt

micellar avg mol wt 80,000, average mol wt 625

packaging

ampule of 5, 5x5, 10x5 mL

Quality Level

200

CMC

0.2-0.9 mM (20-25°C)

transition temp

pour point ~7 °C

solubility

water: soluble

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Show Differences

1 of 4

This Item
X114X102X405
Triton™ X-100 peroxide- and carbonyl-free

Sigma-Aldrich

X100PC

Triton™ X-100

Triton™ X-114 laboratory grade

Sigma-Aldrich

X114

Triton™ X-114

Triton™ X-102

Sigma-Aldrich

X102

Triton™ X-102

Triton™ X-405 solution 70% in H2O

Sigma-Aldrich

X405

Triton™ X-405 solution

form

liquid

form

-

form

-

form

-

aggregation number

100-155

aggregation number

-

aggregation number

-

aggregation number

-

CMC

0.2-0.9 mM (20-25°C)

CMC

0.2 mM (20-25°C)

CMC

0.33 mM

CMC

0.81 mM

mol wt

micellar avg mol wt 80,000, average mol wt 625

mol wt

average mol wt 537

mol wt

757 g/mol

mol wt

~1967.0 g/mol

description

non-ionic

description

non-ionic

description

non-ionic

description

non-ionic

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.[1][2]

Application

0·3% Triton™ X-100 in TBS has been used for permeabilization of retinas before immunostaining.[3][4] 0.5% Triton X-100 has been used for astrocyte cell lysis.[5]

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Packaging

Packaged in ampules under Argon.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

signalword

Danger

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

483.8 °F - closed cup

flash_point_c

251 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2

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Certificates of Analysis (COA)

Lot/Batch Number
0000504594
0000504594
0000488992
0000488992
0000405230

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Cytotoxicity of bilirubin for human fibroblasts and rat astrocytes in culture. Effect of the ratio of bilirubin to serum albumin.
Chuniaud L, et al.
Clinica Chimica Acta; International Journal of Clinical Chemistry, 256(2), 103-114 (1996)
Quantitative ex vivo detection of rodent retinal ganglion cells by immunolabeling Brn-3b.
Experimental Eye Research, 79(1), 131-140 (2004)
Adeno-associated virus mediated SOD gene therapy protects the retinal ganglion cells from chronic intraocular pressure elevation induced injury via attenuating oxidative stress and improving mitochondrial dysfunction in a rat model.
American Journal of Translational Research, 8(2), 799-799 (2016)
The size, shape, and hydration of nonionic surfactant micelles. Triton X-100.
Robson RJ and Dennis EA
The Journal of Physical Chemistry, 81, 1075-1078 (1977)
Friederike Pfeiffer et al.
Acta neuropathologica, 122(5), 601-614 (2011-10-11)
In experimental autoimmune encephalomyelitis (EAE), an animal model for multiple sclerosis (MS), loss of the blood-brain barrier (BBB) tight junction (TJ) protein claudin-3 correlates with immune cell infiltration into the CNS and BBB leakiness. Here we show that sealing BBB
View All Related Papers

Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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