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Triton X-114

laboratory grade

(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol, Polyethylene glycol tert-octylphenyl ether
Linear Formula:
(C2H4O)n C14H22O, n = 7 or 8
CAS Number:
MDL number:
PubChem Substance ID:

Quality Level


laboratory grade



mol wt

average mol wt 537


t-Oct-C6H4-(OCH2CH2)xOH, x = 7-8

refractive index

n20/D 1.488 (lit.)


0.2 mM (20-25°C)

transition temp

pour point ~−9 °C
cloud point 23 °C


1.058 g/mL at 25 °C (lit.)



SMILES string




InChI key


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General description

Triton X-114 is a nonionic detergent. It is a good detergent for solubilizing membranes having reversible phase-separation property useful for the separation of intrinsic membrane proteins. Solution of Triton X-114 is homogeneous at 0°C but phase separation into aqueous phase and a detergent phase occurs above 20°C. Phase separation of proteins in solutions of Triton X-114 has been investigated. This octylphenol ethoxylate is compatible with anionic, cationic, and other nonionic surfactants and chemically stable in most acidic & alkaline solutions.


Triton X-114 has been used:
  • To remove endotoxin from solutions of protein using phase separation technique
  • In the extraction and purification of DNA from endotoxin
  • In the biphasic extraction of lipoproteins from bacterial cell wall
  • As a non-ionic detergent with a low cloud point (23 °C) enabling protein solubilization with phase-partitioning of hydrophilic from amphiphilic proteins


1 gal in poly bottle
5 gal in poly drum
1 L in poly bottle
100, 500 mL in poly bottle

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Signal Word


Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids



Flash Point(F)

483.8 °F

Flash Point(C)

251 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Certificate of Origin

Mechanism and function of a newly identified CpG DNA motif in human primary B cells.
Hartmann G and Krieg A M
Journal of Immunology, 164(2), 944-953 (2000)
Huanhuan Li et al.
Life sciences, 231, 116548-116548 (2019-06-11)
Multidrug resistance is a major obstacle in chemotherapy, which is mainly caused by the overexpression of ATP-binding cassette (ABC) transporters. Breast cancer resistance protein (BCRP) is one of the ABC transporters and is strongly associated with multidrug resistance. Results of
TLR2 and its co-receptors determine responses of macrophages and dendritic cells to lipoproteins of Mycobacterium tuberculosis.
Golenbock D T, et al.
Cellular Immunology, 258(1), 29-37 (2009)
Mycobacterium tuberculosis LprG (Rv1411c): a novel TLR-2 ligand that inhibits human macrophage class II MHC antigen processing.
Gehring A J, et al.
Journal of Immunology, 173(4), 2660-2668 (2004)
Nozomu Takahashi et al.
FEBS open bio, 7(9), 1338-1349 (2017-09-15)
Neuroglobin (Ngb) is a heme protein expressed in the vertebrate brain. We previously engineered a chimeric Ngb protein, in which module M1 of human Ngb is replaced by that of zebrafish Ngb, and showed that the chimeric ZHHH Ngb has

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