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ZZ-100MG-PUR

Sigma-Aldrich

Recombinant Mouse IgG B2

bottle of 100 mg

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Synonym(s):
Antibody Blocker, HAMA, Passive Antibody Blocker, Purified Mouse IgG, Recombinant Mouse IgG

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

material

PETG bottle

clone

recombinant monoclonal

description

Phosphate Buffer Saline

assay

≥90% (HPLC)

form

liquid

packaging

bottle of 100 mg

concentration

~5 mg/mL

technique(s)

ELISA: suitable
immunoblotting: suitable
lateral flow assay: suitable

pH

7.2

isotype

IgG

shipped in

dry ice

storage temp.

−20°C

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This Item
ZZ-10MG-PURA9044A3562
vibrant-m

ZZ-100MG-PUR

Recombinant Mouse IgG B2

vibrant-m

ZZ-10MG-PUR

Recombinant Mouse IgG B2

clone

recombinant monoclonal

clone

recombinant monoclonal

clone

polyclonal

clone

polyclonal

biological source

mouse

biological source

mouse

biological source

rabbit

biological source

goat

technique(s)

ELISA: suitable, lateral flow assay: suitable, immunoblotting: suitable

technique(s)

ELISA: suitable, immunoblotting: suitable, lateral flow assay: suitable

technique(s)

direct ELISA: 1:40,000 using using 5 μg/ml of mouse IgG for coating and OPD substrate, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, western blot (chemiluminescent): 1:80,000-160,000

technique(s)

direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

form

liquid

form

liquid

form

buffered aqueous solution

form

buffered aqueous glycerol solution

General description

Recombinant mouse IgG is a novel, sustainable replacement to serum-derived mouse IgG, providing manufacturers and researchers a tightly-controlled, non-animal-derived blocking solution to efficiently reduce nonspecific antibody interference in diagnostic assays while improving lot reproducibility and test accuracy, yet simplifying import regulations.
The recombinant mouse IgG is offered in two unique blends providing different formulations to select from and optimize for best performance results within a specific immunoassay. The formulas are designed to deliver equivalent or better blocking efficiency compared to serum-derived mouse IgG. This B2 recombinant mouse IgG was developed within a similar formulation as B1 but with directed blocking capability to specifically bind human anti-mouse antibodies present in serum/plasma samples and reduce non-specific binding in an immunoassay system using capture and detection antibodies.  In certain protocols, this directed binding may result in a lower concentration requirement as compared with standard serum-derived mouse IgG.

Features and Benefits

  • Sustainably sourced requiring no animal involvement.
  • Batch reproducibility with clonal-derived, cultured supernatant instead of pooled serum.
  • Security of scalable supply with on-time delivery.
  • Tightly controlled product manufacturing for optimal performance.
  • Simplified import regulations and restrictions.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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25G
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1000309185

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Related Content

The new recombinant mouse IgG antibody provides immunoassay manufacturers a tightly controlled, non-animal derived blocking solution to efficiently reduce nonspecific antibody interference while improving lot reproducibility and test accuracy.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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