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  • 15523
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15523

Sigma-Aldrich

Glycerol

puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, E422, anhydrous, 99.0-101.0% (alkalimetric)

Synonym(s):
1,2,3-Propanetriol, Glycerin
Linear Formula:
HOCH2CH(OH)CH2OH
CAS Number:
Molecular Weight:
92.09
Beilstein:
635685
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.21

vapor density

3.1 (vs air)

Quality Level

vapor pressure

<1 mmHg ( 20 °C)

grade

puriss.

assay

99.0-101.0% (alkalimetric)
99.0-101.0% (calc. on H2O free substance)

form

viscous liquid

autoignition temp.

698 °F

quality

meets analytical specification of Ph. Eur., BP, USP, FCC, E422

expl. lim.

2.6-11.3 %

technique(s)

IR spectroscopy: suitable
electrophoresis: suitable

impurities

acidity or alkalinity, complies
acrolein, glucose and NH4-compounds, complies
aldehydes, in accordance
ester, complies
fatty acids and esters, complies
impurities A and related substances (GC), complies
residual solvents, complies
sugar, complies
≤0.0005% heavy metals (as Pb)
≤0.003% free acid (as CH3COOH)
≤0.003% halogen compounds (as Cl)
≤0.2% 1,2,4-butantriole (GC)
≤2% water (Karl Fischer)

ign. residue

≤0.01% (as SO4)

refractive index

n20/D 1.471-1.474
n20/D 1.474 (lit.)

pH

5.5-8

bp

182 °C/20 mmHg (lit.)

mp

20 °C (lit.)

density

1.2580-1.2644 g/mL at 20 °C
≥1.257 g/mL at 25 °C
1.25 g/mL (lit.)

anion traces

chloride (Cl-): ≤10 mg/kg
sulfate (SO42-): ≤10 mg/kg

cation traces

As: ≤1 mg/kg
Cu: ≤10 mg/kg
Pb: ≤1 mg/kg
Zn: ≤10 mg/kg

SMILES string

OCC(O)CO

suitability

complies for color
complies for reaction against H2SO4
corresponds for chromatography
passes test for identity (IR)

storage temp.

15-25°C

InChI

1S/C3H8O3/c4-1-3(6)2-5/h3-6H,1-2H2

InChI key

PEDCQBHIVMGVHV-UHFFFAOYSA-N

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General description

Glycerol is odourless, colorless, viscous in nature, and exists as a sweet tasting liquid. It can be derived naturally as well as from petrochemical feedstock. Glycerol has a wide variety of applications, and is one of the most valuable and versatile chemical substances in nature. It can be used as an emollient, solvent, sweetening agent, in pharmaceutical formulations, cosmetics, foodstuffs and toiletries. It is very stable and can be easily stored under normal temperature; also it is non-irritating and has no adverse impact to the environment.

Packaging

1, 1, 5, 5, 20 L
25, 70, 250 kg

Application

Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel′s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

Biochem/physiol Actions

Glycerol has been used as
  • a supplement during cell culture of Mycobacterium tuberculosis and Mycobacterium avium.
  • a fuel during designing enzymatic biofuel cell.
  • a liquid composite matrix with 4-HCCA and 3-amino­quinoline for analysis of neutral and acidic glycans.
  • a matrix for fast atom bombardment MS.
  • may be employed as liquid matrix for the quantification studies by MALDI (Matrix-assisted laser desorption/ionization mass spectrometry) analysis.
Glycerol is hygroscopic in nature and is soluble in water owing to its three hydrophilic alcoholic hydroxyl groups. It can form both inter- and intramolecular hydrogen bonds, making it a very flexible molecule. The physiologic effect of glycerine is due to cell-mediated immunity, increased IgG production and increased histamine release.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

390.2 °F - Pensky-Martens closed cup

Flash Point(C)

199 °C - Pensky-Martens closed cup

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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