31437

Sodium bicarbonate

puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99.7%

• Synonym(s): Sodium hydrogen carbonate
• Linear Formula: NaHCO₃
• CAS Number: 144-55-8
• Molecular Weight: 84.01
• Beilstein: 4153970
• EC Number: 205-633-8
• MDL number: MFCD00003528
• PubChem Substance ID: 329753766
• NACRES: NA.85

Properties

• grade: ACS reagent; puriss. p.a.
• Agency: reag. Ph. Eur.
• Assay: ≥99.7%
• form: powder
• impurities: ≤0.0005% ammonium (NH₄); ≤0.003% S-compounds (as SO₄); ≤0.005% I red. matters (as SO₂); ≤0.015% in water insoluble matters
• pH: ≤8.6 (20 °C, 5%)
• pKa (25 °C): (1) 6.37, (2) 10.25 (carbonic acid)
• solubility: H₂O: 0.5 g/10 mL, clear, colorless; water: soluble 50 g/L
• density: 2.16 g/mL at 25 °C (lit.)
• anion traces: chloride (Cl^-): ≤10 mg/kg; phosphate (PO₄^3-): ≤10 mg/kg
• cation traces: As: 2 ppm (MHS-AAS); Ca: ≤100 ppm; Cd: ≤5 ppm; Co: ≤50 ppm; Cu: ≤5 ppm; Fe: ≤5 ppm; K: ≤50 ppm; Mg: ≤50 ppm; Ni: ≤50 ppm; Pb: ≤5 ppm; Zn: ≤5 ppm; heavy metals: ≤5 ppm (by ICP-OES)
• suitability: complies for appearance of solution
• SMILES string: [Na+].OC([O-])=O
• InChI: 1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1
• InChI key: UIIMBOGNXHQVGW-UHFFFAOYSA-M

Description

General description

Sodium bicarbonate is widely used as a physiological buffer. The pKa for bicarbonate ↔ carbonic acid reaction is 6.37. The pKa for bicarbonate ↔ carbonate reaction is 10.25.

Application

Sodium bicarbonate may be used to maintain 7.4pH in Iscove′s modified Dulbecco′s medium for growth of murine hybridoma cell culture. It may be employed as a component of RPMI-1640 liquid medium in a study.