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472476

Sigma-Aldrich

Chloroform

contains amylenes as stabilizer, ACS reagent, ≥99.8%

Synonym(s):
Methylidyne trichloride, Trichloromethane
Empirical Formula (Hill Notation):
CHCl3
CAS Number:
Molecular Weight:
119.38
Beilstein:
1731042
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.04

Quality Level

grade

ACS reagent

vapor density

4.1 (vs air)

vapor pressure

160 mmHg ( 20 °C)

assay

≥99.8%

form

liquid

contains

amylenes as stabilizer

impurities

Acetone and aldehyde, passes test
Acid and chloride, passes test
Free chlorine (Cl), passes test
≤0.03% (water)

evapn. residue

0.001%

color

APHA: ≤10

refractive index

n20/D 1.445 (lit.)

bp

60.5-61.5 °C (lit.)

mp

−63 °C (lit.)

density

1.48 g/mL at 25 °C
1.492 g/mL at 25 °C (lit.)

cation traces

Pb: ≤0.05 ppm

SMILES string

ClC(Cl)Cl

InChI

1S/CHCl3/c2-1(3)4/h1H

InChI key

HEDRZPFGACZZDS-UHFFFAOYSA-N

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Application

Chloroform was used in the process for isolation of RNA from yeast.
It may be used in the following processes:
  • Synthesis of colloidal hydrophobic CdSe core nanocrystals (quantum dots).
  • Preparing phospholipid solutions.
  • As a solvent in the photolysis reaction of ruthenium complexes.
  • Solvent for recrystallization.
  • Reference compound to detect the presence of chloroform in tobacco smokes.
  • Extractant in solvent extraction process.

Packaging

500 mL in glass bottle
1, 2.5, 4×4 L in glass bottle

Pictograms

Skull and crossbonesHealth hazard

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK Germany

WGK 3

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis

Certificate of Origin

A Simple RNA Preparation Procedure from Yeast for Northern Blot Using Hot Phenol.
Wei Y.
Bio-protocol, 2(12) (2012)
Systematic studies on the breakdown of p,p'-DDT in tobacco smokes. Presence of methyl chloride, dichloromethane, and chloroform in tobacco smokes.
Chopra NM and Sherman LR.
Analytical Chemistry, 44(6), 1036-1038 (1972)
Engineering of ultra-small diagnostic nanoprobes through oriented conjugation of single-domain antibodies and quantum dots.
Sukhanova A, et al.
Protocol Exchange (2012)
Kinetics and Mechanism of the Photooxidation of Bis (bipyridine) dichlororuthenium (II) and the Photoreduction of Bis (bipyridine) dichlororuthenium (III) in Chloroform.
Sathiyabalan S and Hoggard PE.
Inorganic Chemistry, 34(18), 4562-4571 (1995)
The affinity of two antimicrobial peptides derived from bovine milk proteins for model lipid membranes.
Barzyk W, et al.
Colloids and Surfaces. A, Physicochemical and Engineering Aspects, 343(1), 104-110 (2009)

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