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47670

Sigma-Aldrich

Formamide

puriss. p.a., ACS reagent, ≥99.5% (GC/T)

Synonym(s):
Amide C1, Formic amide
Linear Formula:
HCONH2
CAS Number:
Molecular Weight:
45.04
Beilstein:
505995
EC Number:
MDL number:
PubChem Substance ID:

grade

ACS reagent
puriss. p.a.

Quality Level

vapor density

1.55 (vs air)

vapor pressure

0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)

Assay

≥99.5% (GC/T)

form

liquid

autoignition temp.

932 °F

expl. lim.

2.7-19 %

technique(s)

PCR: suitable

impurities

≤0.02% free acid (as HCOOH)
≤0.1% water

refractive index

n20/D 1.447 (lit.)
n20/D 1.447

pH

4-10 (20 °C, 200 g/L)

bp

210 °C (lit.)

mp

2-3 °C (lit.)

transition temp

transition temp 2.0-3.0 °C

density

1.132 g/mL at 20 °C
1.134 g/mL at 25 °C (lit.)

cation traces

Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Na: ≤20 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Zn: ≤1 mg/kg

suitability

in accordance for miscibility

SMILES string

NC=O

InChI

1S/CH3NO/c2-1-3/h1H,(H2,2,3)

InChI key

ZHNUHDYFZUAESO-UHFFFAOYSA-N

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1 of 4

This Item
F9037F750347671
Formamide puriss. p.a., ACS reagent, ≥99.5% (GC/T)

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47670

Formamide

Formamide ≥99.5% (GC), BioReagent, for molecular biology

Sigma-Aldrich

F9037

Formamide

Formamide ReagentPlus®, ≥99.0% (GC)

Sigma-Aldrich

F7503

Formamide

Formamide BioUltra, for molecular biology, ≥99.5% (T)

Sigma-Aldrich

47671

Formamide

vapor density

1.55 (vs air)

vapor density

1.55 (vs air)

vapor density

1.55 (vs air)

vapor density

1.55 (vs air)

vapor pressure

0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)

vapor pressure

0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)

vapor pressure

0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)

vapor pressure

0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)

assay

≥99.5% (GC/T)

assay

≥99.5% (GC)

assay

≥99.0% (GC)

assay

≥99.5% (T)

form

liquid

form

liquid

form

liquid

form

liquid

autoignition temp.

932 °F

autoignition temp.

932 °F

autoignition temp.

932 °F

autoignition temp.

932 °F

General description

Formamide is a colorless organic solvent. It has been reported to increase the precision of PCR (Polymerase Chain Reaction) studies. Microwave spectral study of its isotopic forms suggests that the molecule of formamide is nonplanar in which the H2N-C group exhibits a shallow pyramid like structure.

Application

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Carc. 2 - Repr. 1B - STOT RE 2 Oral

Target Organs

Blood

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point(F)

305.6 °F

Flash Point(C)

152 °C


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Microwave spectrum and molecular structure of formamide.
Costain CC and Dowling JM.
J. Chem. Phys. , 32(1), 158-165 (1960)
J Casey et al.
Nucleic acids research, 4(5), 1539-1552 (1977-01-01)
The thermal stabilities of RNA:DNA hybrids are substantially greater than those of DNA:DNA duplexes in aqueous electrolyte solutions containing high concentrations of formamide. Association rates to form DNA:DNA duplexes and DNA:RNA hybrids have been measured in these solvents. There is
Solubilization in formamide protects RNA from degradation.
Chomczynski P.
Nucleic Acids Research, 20(14), 3791-3791 (1992)
The formamide method for the extraction of polysaccharides from haemolytic streptococci.
Fuller AT.
British Journal of Experimental Pathology, 32(1), 130-130 (1960)
Penelope A Bryant et al.
PloS one, 6(5), e19556-e19556 (2011-06-10)
A central issue in the design of microarray-based analysis of global gene expression is that variability resulting from experimental processes may obscure changes resulting from the effect being investigated. This study quantified the variability in gene expression at each level

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