47670

Sigma-Aldrich

Formamide

puriss. p.a., ACS reagent, ≥99.5% (GC/T)

Synonym(s):
Amide C1, Formic amide
Linear Formula:
HCONH2
CAS Number:
Molecular Weight:
45.04
Beilstein/REAXYS Number:
505995
EC Number:
MDL number:
PubChem Substance ID:

Quality Level

grade

ACS reagent
puriss. p.a.

vapor density

1.55 (vs air)

vapor pressure

0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)

assay

≥99.5% (GC/T)

form

liquid

autoignition temp.

932 °F

expl. lim.

2.7-19 %

application(s)

PCR: suitable

impurities

≤0.02% free acid (as HCOOH)
≤0.1% water

refractive index

n20/D 1.447 (lit.)
n20/D 1.447

pH

4-10 (20 °C, 200 g/L)

bp

210 °C (lit.)

mp

2-3 °C (lit.)

transition temp

transition temp 2.0-3.0 °C

density

1.132 g/mL at 20 °C
1.134 g/mL at 25 °C (lit.)

cation traces

Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Na: ≤20 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Zn: ≤1 mg/kg

suitability

in accordance for miscibility

SMILES string

NC=O

InChI

1S/CH3NO/c2-1-3/h1H,(H2,2,3)

InChI key

ZHNUHDYFZUAESO-UHFFFAOYSA-N

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General description

Formamide is a colorless organic solvent. It has been reported to increase the precision of PCR (Polymerase Chain Reaction) studies. Microwave spectral study of its isotopic forms suggests that the molecule of formamide is nonplanar in which the H2N-C group exhibits a shallow pyramid like structure.

Packaging

1, 2.5 L in glass bottle
4×25, 250 mL in glass bottle

Application

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

hazcat

Carc. 2 - Repr. 1B - STOT RE 2 Oral

Target Organs

Blood

storage_class_code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK Germany

WGK 1

Flash Point(F)

305.6 °F

Flash Point(C)

152 °C

Certificate of Analysis

Certificate of Origin

J Casey et al.
Nucleic acids research, 4(5), 1539-1552 (1977-01-01)
The thermal stabilities of RNA:DNA hybrids are substantially greater than those of DNA:DNA duplexes in aqueous electrolyte solutions containing high concentrations of formamide. Association rates to form DNA:DNA duplexes and DNA:RNA hybrids have been measured in these solvents. There is...
The formamide method for the extraction of polysaccharides from haemolytic streptococci.
Fuller AT.
British Journal of Experimental Pathology, 32(1), 130-130 (1960)
Solubilization in formamide protects RNA from degradation.
Chomczynski P.
Nucleic Acids Research, 20(14), 3791-3791 (1992)
Formamide can dramatically improve the specificity of PCR.
G Sarkar et al.
Nucleic acids research, 18(24), 7465-7465 (1990-12-25)
Microwave spectrum and molecular structure of formamide.
Costain CC and Dowling JM.
J. Chem. Phys. , 32(1), 158-165 (1960)
Protocols
GC Analysis of Class 2 Residual Solvents on OVI-G43
Read More

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