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650471

Sigma-Aldrich

Chloroform

HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains 0.5-1.0% ethanol as stabilizer

Synonym(s):
Methylidyne trichloride, Trichloromethane
Empirical Formula (Hill Notation):
CHCl3
CAS Number:
Molecular Weight:
119.38
Beilstein:
1731042
MDL number:
PubChem Substance ID:
NACRES:
NA.04

grade

HPLC Plus
for residue analysis

Quality Level

vapor density

4.1 (vs air)

vapor pressure

160 mmHg ( 20 °C)

Assay

≥99.9%

form

liquid

contains

0.5-1.0% ethanol as stabilizer

technique(s)

HPLC: suitable
UV/Vis spectroscopy: suitable
gas chromatography (GC): suitable

impurities

≤0.5 ppb Fluorescence (quinine) at 365 nm
<0.02% water

evapn. residue

<0.0001%

halogenated residue

<10 ng/L (as heptachlor epoxide)

refractive index

n20/D 1.445 (lit.)

bp

60.5-61.5 °C (lit.)

mp

−63 °C (lit.)

density

1.48 g/mL at 25 °C
1.492 g/mL at 25 °C (lit.)

λ

H2O reference

UV absorption

λ: 245 nm Amax: 1.00
λ: 250 nm Amax: 0.30
λ: 275-400 nm Amax: 0.005

application(s)

food and beverages

SMILES string

ClC(Cl)Cl

InChI

1S/CHCl3/c2-1(3)4/h1H

InChI key

HEDRZPFGACZZDS-UHFFFAOYSA-N

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1 of 4

This Item
439142650498366927
Chloroform HPLC Plus, for HPLC, GC, and residue analysis, &#8805;99.9%, contains 0.5-1.0% ethanol as stabilizer

Sigma-Aldrich

650471

Chloroform

Chloroform suitable for HPLC, &#8805;99.8%, contains 0.5-1.0% ethanol as stabilizer

Supelco

439142

Chloroform

Chloroform HPLC Plus, for HPLC, GC, and residue analysis, &#8805;99.9%, contains amylenes as stabilizer

Sigma-Aldrich

650498

Chloroform

Chloroform suitable for HPLC, &#8805;99.8%, contains 0.5-1.0% ethanol as stabilizer

Sigma-Aldrich

366927

Chloroform

vapor density

4.1 (vs air)

vapor density

4.1 (vs air)

vapor density

4.1 (vs air)

vapor density

4.1 (vs air)

vapor pressure

160 mmHg ( 20 °C)

vapor pressure

160 mmHg ( 20 °C)

vapor pressure

160 mmHg ( 20 °C)

vapor pressure

160 mmHg ( 20 °C)

assay

≥99.9%

assay

≥99.8%

assay

≥99.9%

assay

≥99.8%

form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

HPLC: suitable, UV/Vis spectroscopy: suitable, gas chromatography (GC): suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

HPLC: suitable

Application

High-purity, multipurpose solvent, tested for suitability in:
  • HPLC
  • Spectrophotometry
  • Residual testing
  • LC-MS applications

Used as a solvent in:
  • Extraction of anthracyclines and metabolites prior to analysis by liquid chromatography with fluorescence detection
  • Extraction and handling of phospholipids from biological samples prior to electrospray ionization mass spectrometry and LC–MS determinations

Suitable for HPLC, spectrophotometry, environmental testing

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Pictograms

Skull and crossbonesHealth hazard

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Flash Point(F)

does not flash

Flash Point(C)

does not flash


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Traces of phosgene in chloroform: Consequences for extraction of anthracyclines.
Maudens KE, et al.
Journal of Chromatography. B, Biomedical Applications, 848(2), 384-390 (2007)
Systematic studies on the breakdown of p,p'-DDT in tobacco smokes. Presence of methyl chloride, dichloromethane, and chloroform in tobacco smokes.
Chopra NM and Sherman LR.
Analytical Chemistry, 44(6), 1036-1038 (1972)
The affinity of two antimicrobial peptides derived from bovine milk proteins for model lipid membranes.
Barzyk W, et al.
Colloids and Surfaces. A, Physicochemical and Engineering Aspects, 343(1), 104-110 (2009)
Kinetics and Mechanism of the Photooxidation of Bis (bipyridine) dichlororuthenium (II) and the Photoreduction of Bis (bipyridine) dichlororuthenium (III) in Chloroform.
Sathiyabalan S and Hoggard PE.
Inorganic Chemistry, 34(18), 4562-4571 (1995)
Engineering of ultra-small diagnostic nanoprobes through oriented conjugation of single-domain antibodies and quantum dots.
Sukhanova A, et al.
Protocol Exchange (2012)

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