Some of the Protease Inhibitor Cocktail products that we offer are already in solution in DMSO, and they can be further diluted in water or aqueous buffer. The Protease Inhibitor Cocktail products that we offer as a solid (that is, in powder or tablet form) can be directly dissolved in water. So there should not be a need to use a DMSO such as this product to solubilize a protease inhibitor cocktail from our company. But having said that, it is possible that other companies may offer a DMSO-soluble form...in which case, this DMSO would be fine to use.
Please follow the manufacturer's recommendations regarding storage of protease inhibitor cocktail solutions; note that DMSO (and solutions in DMSO) will freeze solid at -20°C.
D5879
Dimethyl sulfoxide
ReagentPlus®, ≥99.5%
Synonym(s):
DMSO
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About This Item
Linear Formula:
(CH3)2SO
CAS Number:
Molecular Weight:
78.13
UNSPSC Code:
12191502
NACRES:
NA.21
PubChem Substance ID:
EC Number:
200-664-3
Beilstein/REAXYS Number:
506008
MDL number:
Assay:
≥99.5%
Technique(s):
immunoblotting: suitable
Bp:
189 °C (lit.)
Vapor pressure:
0.42 mmHg ( 20 °C)
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InChI key
IAZDPXIOMUYVGZ-UHFFFAOYSA-N
InChI
1S/C2H6OS/c1-4(2)3/h1-2H3
SMILES string
CS(C)=O
Quality Level
vapor density
2.7 (vs air)
vapor pressure
0.42 mmHg ( 20 °C)
product line
ReagentPlus®
assay
≥99.5%
form
liquid
autoignition temp.
573 °F
expl. lim.
42 %, 63 °F
technique(s)
immunoblotting: suitable
impurities
≤0.1% water (Karl Fischer)
refractive index
n20/D 1.479 (lit.)
bp
189 °C (lit.)
mp
16-19 °C (lit.)
solubility
H2O: soluble(lit.), acetone: soluble(lit.), benzene: soluble(lit.), chloroform: soluble(lit.), ethanol: soluble(lit.)
density
1.10 g/mL (lit.)
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Related Categories
1 of 4
This Item | |||
|---|---|---|---|
| assay ≥99.5% | assay ≥99.9% | assay ≥99.9% (GC) | assay ≥99.5% (GC) |
| technique(s) immunoblotting: suitable | technique(s) - | technique(s) - | technique(s) - |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 200 |
| solubility H2O: soluble(lit.), benzene: soluble(lit.), ethanol: soluble(lit.), acetone: soluble(lit.), chloroform: soluble(lit.) | solubility - | solubility - | solubility H2O: miscible (completely) |
| form liquid | form liquid | form liquid | form liquid |
| density 1.10 g/mL (lit.) | density 1.10 g/mL (lit.) | density 1.10 g/mL (lit.) | density 1.10 g/mL (lit.) |
General description
Application
DMSO has been used as a medium to prepare a chemiluminescence reagent that can be a substitute to commercially-available enhanced chemiluminescence kits for use in immunoblotting technique.[4]
Biochem/physiol Actions
Packaging
Note: D5879-1L in plastic jerricans
Other Notes
For information on dimethyl sulfoxide miscibility, please visit the following link:
Dimethyl Sulfoxide Miscibility/Immiscibility Table
Dimethyl Sulfoxide Miscibility/Immiscibility Table
Legal Information
ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.
flash_point_f
188.6 °F - closed cup
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_c
87 °C - closed cup
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Lowe, O.G
The Journal of Organic Chemistry, 41 (1976)
Activated dimethyl sulfoxide: useful reagents for synthesis.
Mancuso AJ and Swern D.
Synthesis, 1981(03), 165-185 (1981)
Levent Keskintepe et al.
Journal of assisted reproduction and genetics, 26(11-12), 629-635 (2009-12-08)
Cryopreservation of blastocysts, especially those subjected to the trauma due to blastomere biopsy for the purposes of pre-implantation genetic screening (PGS), requires significant optimization. Laboratory and clinical outcomes were compared to determine the effect of two different cryopreservation techniques on
Sun-A Ock et al.
Cell transplantation, 20(8), 1231-1239 (2011-02-08)
Dimethyl sulfoxide (DMSO), a commonly used cryoprotectant in cryopreservation procedures, is detrimental to viability of cells. In this view point, a comparative study was carried out to evaluate the effect of DMSO on porcine mesenchymal stem cells (pMSCs). We compared
Julia L Zehr et al.
Biology of reproduction, 72(5), 1087-1094 (2004-12-31)
This study investigated whether prenatal androgen exposure, social rank, and body weight are factors regulating pubertal development in outdoor-housed female rhesus monkeys. Subjects' mothers received injections of testosterone enanthate (20 mg/ wk), flutamide (an androgen receptor blocker, 30 mg/kg twice
Related Content
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Can I use this D5879 to solubilise protease inhibitor cocktail, and will store it at -20 °C?
1 answer-
Helpful?
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Is this product sterile?
1 answer-
This item is not a sterile preparation. Please see the links below to review available sterile DMSO product options:
D2650
https://www.sigmaaldrich.com/product/sigma/d2650
D2438
https://www.sigmaaldrich.com/product/sigma/d2438Helpful?
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
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Is the DMSO (Dimethyl Sulfoxide) still considered usable if it was received in a frozen or solid form?
1 answer-
Yes, DMSO (Dimethyl Sulfoxide) is still considered usable even if it was received in a frozen or solid form. The solidified DMSO can be easily re-liquefied by warming it to room temperature.
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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The Dimethyl sulfoxide, Product D5879, I ordered arrived as a solid. Is the product still acceptable?
1 answer-
Sigma-Aldrich ships many products, including DMSO, at ambient temperature. As a result product D5879 might arrive as a solid depending on the weather conditions during shipment. The melting point of DMSO is 16-19°C. Therefore, if the solid is allowed to warm to room temperature, it will melt without any impact on the quality of the product.
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What is the molarity of Product D5879, Dimethyl sulfoxide?
1 answer-
Pure solvents do not technically have a molarity (defined as moles of solute per liter of solution), since there is no solute distinct from the pure material. However, an effective molar concentration of 14.1M can be determined for this product, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.
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My application requires a dry solvent. How can I remove water that is present in Dimethyl sulfoxide, Product D5879?
1 answer-
This product has a water content specification of not more than 0.1%, which may be low enough for your application. Other DMSO products may have more water present. Molecular sieves are typically zeolite compounds that strongly adsorb water and have carefully controlled pore sizes. If you would like, dried sieves may be placed into the bulk solvent container (about 5%-10% by volume) to remove most water. Sigma-Aldrich offers 4 Å molecular sieves (e.g. product 334308), which would be effective in removing residual water from DMSO.
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