F7503

Sigma-Aldrich

Formamide

ReagentPlus®, ≥99.0% (GC)

Synonym(s):
Amide C1, Formic amide
Linear Formula:
HCONH2
CAS Number:
Molecular Weight:
45.04
Beilstein/REAXYS Number:
505995
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.21

Quality Level

vapor density

1.55 (vs air)

vapor pressure

0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)

product line

ReagentPlus®

assay

≥99.0% (GC)

form

liquid

autoignition temp.

932 °F

expl. lim.

2.7-19 %

application(s)

hybridization: suitable

refractive index

n20/D 1.447 (lit.)

pH

4-10 (20 °C, 200 g/L)

bp

210 °C (lit.)

mp

2-3 °C (lit.)

density

1.134 g/mL at 25 °C (lit.)

SMILES string

NC=O

InChI

1S/CH3NO/c2-1-3/h1H,(H2,2,3)

InChI key

ZHNUHDYFZUAESO-UHFFFAOYSA-N

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Related Categories

Packaging

100, 250, 500 mL in glass bottle
2.5, 4 L in poly bottle
1 L in glass bottle

Application

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Legal Information

ReagentPlus is a registered trademark of Sigma-Aldrich Co. LLC

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

hazcat

Carc. 2 - Repr. 1B - STOT RE 2 Oral

Target Organs

Blood

storage_class_code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK Germany

WGK 1

Flash Point(F)

305.6 °F

Flash Point(C)

152 °C

Certificate of Analysis

Certificate of Origin

The crystal structure of formamide.
Ladell J and Post B.
Acta Crystallographica, 7(8-9), 559-564 (1954)
The formamide method for the extraction of polysaccharides from haemolytic streptococci.
Fuller AT.
British Journal of Experimental Pathology, 19(2), 130-130 (1938)
Microwave spectrum, structure, dipole moment, and quadrupole coupling constants of formamide.
Kurland RJ and Wilson Jr EB.
J. Chem. Phys. , 27(2), 585-590 (1957)
Formamide can dramatically improve the specificity of PCR.
G Sarkar et al.
Nucleic acids research, 18(24), 7465-7465 (1990-12-25)
The dynamics of liquid formamide, N-methylformamide, N,N-dimethylformamide, and N,N-dimethylacetamide. A dielectric relaxation study.
Barthel J, et al.
Journal of Molecular Liquids, 98, 51-69 (2002)
Protocols
GC Analysis of Class 2 Residual Solvents on OVI-G43
Read More

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