01632

Atto 520-Biotin

BioReagent, suitable for fluorescence, ≥90% (HPLC)

• Synonym(s): Biotin-Atto 520
• Empirical Formula (Hill Notation): C₃₇H₅₃ClN₆O₈S
• Molecular Weight: 777.37
• MDL number: MFCD04040671
• eCl@ss: 34058011
• PubChem Substance ID: 329747336
• NACRES: NA.32

Properties

• product line: BioReagent
• Quality Level: 100
• assay: ≥90% (HPLC)
• form: powder
• manufacturer/tradename: ATTO-TEC GmbH
• solubility: DMF: soluble; ethanol: soluble; methanol: soluble
• λ: in ethanol (with 0.1% trifluoroacetic acid)
• UV absorption: λ: 522-528 nm A_max
• suitability: suitable for fluorescence
• storage temp.: 2-8°C
• SMILES string: [O-]Cl(=O)(=O)=O.CCNc1cc2OC3=CC(=[NH+]\CC)\C(C)=CC3=C(CCC(=O)NCCCCCNC(=O)CCCCC4SCC5NC(=O)NC45)c2cc1C
• InChI: 1S/C37H52N6O4S.ClHO4/c1-5-38-28-20-31-26(18-23(28)3)25(27-19-24(4)29(39-6-2)21-32(27)47-31)14-15-35(45)41-17-11-7-10-16-40-34(44)13-9-8-12-33-36-30(22-48-33)42-37(46)43-36;2-1(3,4)5/h18-21,30,33,36,38H,5-17,22H2,1-4H3,(H,40,44)(H,41,45)(H2,42,43,46);(H,2,3,4,5)/b39-29-;
• InChI key: OCYRRKMPTKPPHX-QITHAKLPSA-N

Description

Application

Atto 520 is a fluorescent label with high molecular absorption (110.000) and quantum yield (0.90) as well as sufficient stoke′s shift (excitation maximum 520 nm, emission maximum 542 nm). It does nearly not show cis-trans-isomerisation, which limits brightness and reproducibility for many other dyes. Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Can be utilized in applications such as ELISA or immuno-histochemistry, in situ hybridization, flow cytometry and others, to identify streptavidin, avidin, or extravidin-conjugates. Atto-520 fluorescent-labeled biotin can be loaded onto magnetic beads coated with streptavidin through specific ligand–receptor interactions.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves