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01632

Sigma-Aldrich

Atto 520-Biotin

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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Synonym(s):
Biotin-Atto 520
Empirical Formula (Hill Notation):
C37H53ClN6O8S
Molecular Weight:
777.37
MDL number:
eCl@ss:
34058011
PubChem Substance ID:
NACRES:
NA.32

product line

BioReagent

Assay

≥90% (HPLC)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

solubility

DMF: soluble
ethanol: soluble
methanol: soluble

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 522-528 nm Amax

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

[O-]Cl(=O)(=O)=O.CCNc1cc2OC3=CC(=[NH+]\CC)\C(C)=CC3=C(CCC(=O)NCCCCCNC(=O)CCCCC4SCC5NC(=O)NC45)c2cc1C

InChI

1S/C37H52N6O4S.ClHO4/c1-5-38-28-20-31-26(18-23(28)3)25(27-19-24(4)29(39-6-2)21-32(27)47-31)14-15-35(45)41-17-11-7-10-16-40-34(44)13-9-8-12-33-36-30(22-48-33)42-37(46)43-36;2-1(3,4)5/h18-21,30,33,36,38H,5-17,22H2,1-4H3,(H,40,44)(H,41,45)(H2,42,43,46);(H,2,3,4,5)/b39-29-;

InChI key

OCYRRKMPTKPPHX-QITHAKLPSA-N

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Atto 520-Biotin BioReagent, suitable for fluorescence, ≥90% (HPLC)

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Atto 647N-Biotin

assay

≥90% (HPLC)

assay

≥90% (HPCE)

assay

≥90.0% (HPLC)

assay

≥90.0% (HPLC)

form

powder

form

powder

form

-

form

powder

solubility

DMF: soluble, ethanol: soluble, methanol: soluble

solubility

DMF: soluble, DMSO: soluble, acetonitrile: soluble

solubility

-

solubility

-

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Application

Atto 520 is a fluorescent label with high molecular absorption (110.000) and quantum yield (0.90) as well as sufficient stoke′s shift (excitation maximum 520 nm, emission maximum 542 nm). It does nearly not show cis-trans-isomerisation, which limits brightness and reproducibility for many other dyes. Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Can be utilized in applications such as ELISA or immuno-histochemistry, in situ hybridization, flow cytometry and others, to identify streptavidin, avidin, or extravidin-conjugates. Atto-520 fluorescent-labeled biotin can be loaded onto magnetic beads coated with streptavidin through specific ligand–receptor interactions.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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