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02065

Adenosine 5′-triphosphate, immobilized on Agarose 4B

suitable for affinity chromatography, powder (lyophilized)

Synonym(s):

5′-ATP-agarose 4B

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$128.10

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$2,872.50

$128.10

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About This Item

UNSPSC Code:
41106305
eCl@ss:
32160414
PubChem Substance ID:
24845221
NACRES:
NA.51

Key Documents

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form

powder (lyophilized)

contains

60% lactose as stabilizer

technique(s)

affinity chromatography: suitable

capacity

≥1 μmol/mL, packed gel capacity (5′-ATP)(bound by a C6-spacer to C-8 of ATP)

storage temp.

−20°C

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1 of 4

This Item
A2767A2810A0144
Sigma-Aldrich

Sigma-Aldrich

02065

Adenosine 5′-triphosphate, immobilized on Agarose 4B

Adenosine 5′-triphosphate–Agarose lyophilized powder

Sigma-Aldrich

A2767

Adenosine 5′-triphosphate–Agarose

Adenosine 5′-diphosphate–Agarose lyophilized powder

Sigma-Aldrich

A2810

Adenosine 5′-diphosphate–Agarose

Adenosine 3′,5′-cyclic monophosphate–Agarose lyophilized powder

Sigma-Aldrich

A0144

Adenosine 3′,5′-cyclic monophosphate–Agarose

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

powder (lyophilized)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

contains

60% lactose as stabilizer

contains

-

contains

-

contains

-

technique(s)

affinity chromatography: suitable

technique(s)

-

technique(s)

-

technique(s)

-

capacity

≥1 μmol/mL, packed gel capacity (5′-ATP)(bound by a C6-spacer to C-8 of ATP)

capacity

-

capacity

-

capacity

-

General description

The lactose-stabilizer must be removed prior to use by washing the gel onto filter with water or buffer.

Application

Adenosine 5′-triphosphate, immobilized on Agarose 4B (5′-ATP-agarose 4B) is intended for use in affinity chromatography. 5′-ATP-agarose 4B has been shown to bind enzymes with affinity to 5′-ATP. 5′-ATP-agarose 4B may be considered for used with enzymes that bind to other ATP-agarose and sepharose affinity media or beads.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Purification of cofactor-dependent enzymes by affinity chromatography[1][2].

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCBV4900
BCBS5608V
BCBR7479V
BCBM7177V
BCBK5530V

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Purification of cofactor-dependent enzymes by affinity chromatography.
Lee C-Y et al.
Analytical biochemistry, 77(1), 90-102 (1977-01-01)
Zhengyu Yin et al.
Biochemistry, 48(2), 336-345 (2008-12-31)
(-)-Epigallocatechin-3-gallate (EGCG), a major component of green tea, protects against certain types of cancers, although the mechanism has not yet been determined. It was previously demonstrated that EGCG blocks aryl hydrocarbon receptor (AhR)-mediated transcription induced by the potent carcinogen 2,3,7,8-tetrachlorodibenzo-p-dioxin
E Roggen et al.
European journal of biochemistry, 147(2), 225-232 (1985-03-01)
Poly(A) polymerase has been purified to near homogeneity from the cytoplasm of Artemia salina cryptobiotic gastrulae by ion-exchange chromatography on DEAE-cellulose, DEAE-Sepharose CL-6B and phosphocellulose P11, gel filtration on CL-Sepharose 6B, affinity chromatography on poly(A)-Sepharose 4B and ATP-agarose. The enzyme
G A Nevinsky et al.
Applied biochemistry and biotechnology, 75(1), 77-91 (1999-04-24)
This article presents evidence that protein kinase activity is an intrinsic property of secretory immunoglobulin A (sIgA) from milk of healthy human mothers. Polyclonal sIgA was purified by sequential chromatography on protein A-Sepharose, DEAE-cellulose, and gel filtration on Toyopearl HW-55
ATP-and dATP-substituted agaroses and the purification of ribonucleotide reductases.
O Berglund et al.
Methods in enzymology, 34, 253-261 (1974-01-01)
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