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02744

Sigma-Aldrich

Atto 655-Streptavidin

BioReagent, suitable for fluorescence

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Synonym(s):
Streptavidin-Atto 655
NACRES:
NA.32

product line

BioReagent

Quality Level

100

form

powder

manufacturer/tradename

ATTO-TEC GmbH

solubility

DMF: soluble
DMSO: soluble
acetonitrile: soluble

fluorescence

λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence
suitable for gel electrophoresis

storage temp.

−20°C

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Atto 655-Streptavidin

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06966

Atto 655-Biotin

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form

powder

form

powder

form

solid

form

powder

solubility

DMF: soluble, DMSO: soluble, acetonitrile: soluble

solubility

DMF: soluble, DMSO: soluble, acetonitrile: soluble

solubility

-

solubility

-

fluorescence

λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 554 nm; λem 576 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 590 nm; λem 619 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence, suitable for gel electrophoresis

suitability

suitable for fluorescence

suitability

-

suitability

suitable for fluorescence

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Application

Atto 655 is a fluorescent label with high molecular absorption (125.000) and quantum yield (0.30), as well as sufficient stoke′s shift (excitation maximum 663 nm, emission maximum 684 nm). There is very little cis-trans-isomerisation, which limits brightness and reproducibility for many other dyes. Atto 655 has been coupled to streptavidin to enable various types of assays based on the specific affinity of biotin to streptavidin. Streptavidin conjugates are used as secondary detection reagents in histochemical applications, flow cytometry, microarrays, blot analysis and immunoassays.

Analysis Note

free of unconjugated dye

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Breaking the Diffraction Barrier in Fluorescence Microscopy by Optical Shelving.
Bretschneider, S.; Eggeling, Chr.; Hell, S. W.
Physical Review Letters, 98(21) (2007)
Triple-Color Super-Resolution Imaging of Live Cells. Resolving Submicroscopic Receptor Organization in the Plasma Membrane.
Wilmes, S.; Staufenbiel, M.; Lisse, D.; Richter, Ch. P.; Beutel, O.; Busch, K. B. et al.
Angewandte Chemie (International Edition in English), 124(20), 4952-4955 (2012)
Simple buffers for 3D STORM microscopy.
van de Linde, S.; Kasper, R.; Heilemann, M.; Sauer, M.
Applied Physics B: Lasers and Optics, 93(4), 725-731 (2008)
Nicolas Olivier et al.
Biomedical optics express, 4(6), 885-899 (2013-06-14)
3D STORM is one of the leading methods for super-resolution imaging, with resolution down to 10 nm in the lateral direction, and 30-50 nm in the axial direction. However, there is one important requirement to perform this type of imaging:
PALM and STORM. Unlocking live-cell super-resolution.
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