Atto 665 NHS ester

Name: Atto 665 NHS ester
Brand: SIGMA

BioReagent, suitable for fluorescence

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About This Item

UNSPSC Code:

12352108

NACRES:

NA.32

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Key Documents

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product line

BioReagent

assay

≥90% (coupling to amines)

mol wt

M_w 820 g/mol

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λ_ex 663 nm; λ_em 684 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

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This Item	18373	78362	93516
Sigma-Aldrich 04022 Atto 665 NHS ester	Sigma-Aldrich 18373 Atto 647N NHS ester	Sigma-Aldrich 78362 Atto 490LS NHS ester	Sigma-Aldrich 93516 Atto Rho6G NHS ester
suitability suitable for fluorescence	suitability suitable for fluorescence	suitability suitable for fluorescence	suitability suitable for fluorescence
fluorescence λ_ex 663 nm; λ_em 684 nm in 0.1 M phosphate pH 7.0	fluorescence -	fluorescence -	fluorescence -
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 100	Quality Level 100	Quality Level 100	Quality Level 100
assay ≥90% (coupling to amines)	assay ≥90% (HPLC), ≥90% (degree of coupling)	assay ≥90% (HPLC), ≥90% (degree of coupling)	assay ≥90% (HPLC)
product line BioReagent	product line BioReagent	product line BioReagent	product line -

General description

Atto 665 is a new fluorescent label closely related to Atto 647N. It shows extraordinary high fluorescence quantum yield, excellent thermal and photo-stability, outstanding ozone resistance, and very little triplet formation. Atto 665 is a cationic (charge ⁺¹). The dye is moderately hydrophilic. The NHS-ester of the dye reacts easily with amino-groups of proteins and other bio-molecules. Since the amino group must be non protonated to be reactive, the pH of the reaction is recommended to be about 8.3. Prepare labeling solution of NHS-ester immediately before use by dissolving the vial content in anhydrous and amine-free DMF or DMSO.

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Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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