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04259

N-Ethylmaleimide

BioUltra, ≥99.0% (HPLC)

Synonym(s):

NEM

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5 g

Available to ship TODAYfromMILWAUKEE

$199.00
25 g

Available to ship TODAYfromMILWAUKEE

$776.00

About This Item

Empirical Formula (Hill Notation):
C6H7NO2
CAS Number:
Molecular Weight:
125.13
NACRES:
NA.26
PubChem Substance ID:
UNSPSC Code:
12352106
EC Number:
204-892-4
MDL number:
Beilstein/REAXYS Number:
112448

$199.00


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product line

BioUltra

Quality Level

assay

≥99.0% (HPLC)

impurities

insoluble matter, passes filter test

ign. residue

≤0.05%

bp

210 °C (lit.)

mp

43-46 °C (lit.), 44-47 °C

solubility

methanol: 1 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤50 mg/kg, sulfate (SO42-): ≤100 mg/kg

cation traces

Al: ≤5 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

absorption

cut-off at 382 nm in methanol at 1 M

storage temp.

2-8°C

SMILES string

CCN1C(=O)C=CC1=O

InChI

1S/C6H7NO2/c1-2-7-5(8)3-4-6(7)9/h3-4H,2H2,1H3

InChI key

HDFGOPSGAURCEO-UHFFFAOYSA-N

Gene Information

Application

N-Ethylmaleimide (NEM) has been used:
  • to prepare NEM-modified myosin beads
  • to block unmodified free thiols in samples
  • as a supplement in IP lysis/wash buffer for in vitro SUMOylation assay

Reagent for the covalent modification of cysteine residues in proteins.

Biochem/physiol Actions

Augments currents from native M-channels in sympathetic neurons and acts as an opener for KCNQ2, KCNQ4 and KCNQ5 channels.
Sulfhydryl alkylating agent that inactivates NADP-dependent isocitrate dehydrogenase and many endonucleases.

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This Item
04260E127134115-M
assay

≥99.0% (HPLC)

assay

≥99.0% (HPLC)

assay

≥98% (HPLC)

assay

≥98% (HPLC)

product line

BioUltra

product line

-

product line

BioXtra

product line

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

solubility

methanol: 1 M at 20 °C, clear, colorless

solubility

-

solubility

95% ethanol: 50 mg/mL, clear to slightly hazy (colorless to faint yellow)

solubility

ethanol: 5 mg/mL

mp

43-46 °C (lit.), 44-47 °C

mp

43-46 °C

mp

43-46 °C (lit.)

mp

-

impurities

insoluble matter, passes filter test

impurities

-

impurities

≤0.001% Phosphorus (P), ≤0.1% Insoluble matter

impurities

-


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pictograms

Skull and crossbonesCorrosion

signalword

Danger

Hazard Classifications

Acute Tox. 2 Oral - Acute Tox. 3 Dermal - Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

flash_point_f

163.2 °F - closed cup

flash_point_c

72.9 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges



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SUMOylation of periplakin is critical for efficient reorganization of keratin filament network
Gujrati M, et al.
Molecular Biology of the Cell, 30(3), 357-369 (2019)
Measuring the kinetic and mechanical properties of non-processive myosins using optical tweezers
Greenberg MJ, et al.
Methods in Molecular Biology, 483-509 (2017)
Resin-assisted enrichment of thiols as a general strategy for proteomic profiling of cysteine-based reversible modifications
Jia Guo
Nature Protocols, 9(1), 64-75 (2014)



Global Trade Item Number

SKUGTIN
212970-50G04061832928678
212970-10G04061832928661
212970-1G04061826171202
04259-25G04061833416402
04259-5G04061838629296

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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