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05066

Sigma-Aldrich

Agarose

High EEO, for molecular biology

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Synonym(s):
3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose HE
CAS Number:
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

Quality Level

form

powder

impurities

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

ign. residue

≤1%

loss

≤10% loss on drying

EEO

0.23-0.27

transition temp

gel point 34-37 °C (1.5% solution)

gel strength

≥1500 g/cm2

anion traces

chloride (Cl-): ≤0.3%
sulfate (SO42-): ≤0.6%

cation traces

Ca: ≤0.05%
Cd: ≤0.001%
Co: ≤0.001%
Cr: ≤0.001%
Cu: ≤0.001%
Fe: ≤0.001%
K: ≤0.05%
Mg: ≤0.001%
Mn: ≤0.001%
Na: ≤0.5%
Ni: ≤0.001%
Pb: ≤0.001%
Zn: ≤0.001%

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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This Item
A5030A3643A9918
Agarose High EEO, for molecular biology

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05066

Agarose

Agarose Ultra-low Gelling Temperature

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A5030

Agarose

Agarose Type IV, Special High EEO

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Agarose Type II-A, Medium EEO

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A9918

Agarose

form

powder

form

powder

form

powder

form

powder

impurities

DNases, none detected

impurities

-

impurities

-

impurities

≤7% water

ign. residue

≤1%

ign. residue

-

ign. residue

-

ign. residue

-

loss

≤10% loss on drying

loss

-

loss

-

loss

-

EEO

0.23-0.27

EEO

≤0.05

EEO

≥0.3

EEO

0.16-0.19

Application

Agarose is used for preparative and analytical separation of nucleic acids. It can be used for single cell gel electrophoresis assay. It can also be used for southern blotting. It can also be used for assessing transepithelial ionic fluxes from cultured neonatal rat semicircular canal epithelium.

Biochem/physiol Actions

Agarose is a polysaccharide used for resolving DNA and RNA fragments from 500 - 20,000 bp. It provides strong gel structure which assists in better handling and less breakage. It can also reexpress the differentiated collagen phenotype from dedifferentiated chondrocytes during agarose gel culture.

Packaging

Packaging
50, 500 g in poly bottle

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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K C Reed et al.
Nucleic acids research, 13(20), 7207-7221 (1985-10-25)
The unique properties of nylon membranes allow for dramatic improvement in the capillary transfer of DNA restriction fragments from agarose gels (Southern blotting). By using 0.4 M NaOH as the transfer solvent following a short pre-treatment of the gel in
C M Hughes et al.
Molecular human reproduction, 2(8), 613-619 (1996-08-01)
Baseline DNA damage in spermatozoa from fertile and infertile men was compared using a modified alkali single cell gel electrophoresis (comet) assay. Semen from normozoospermic fertile, normozoospermic infertile and asthenozoospermic infertile (World Health Organization criteria, 1992) samples were studied. No
Pierre G Milhaud et al.
American journal of physiology. Cell physiology, 283(6), C1752-C1760 (2002-10-22)
The ductal epithelium of the semicircular canal forms much of the boundary between the K+-rich luminal fluid and the Na+-rich abluminal fluid. We sought to determine whether the net ion flux producing the apical-to-basal short-circuit current (I(sc)) in primary cultures
P D Benya et al.
Cell, 30(1), 215-224 (1982-08-01)
The differentiated phenotype of rabbit articular chondrocytes consists primarily of type II collagen and cartilage-specific proteoglycan. During serial monolayer culture this phenotype is lost and replaced by a complex collagen phenotype consisting predominately of type I collagen and a low
Aurica Precupas et al.
Chemical research in toxicology, 33(8), 2054-2071 (2020-07-01)
Understanding nanomaterial (NM)-protein interactions is a key issue in defining the bioreactivity of NMs with great impact for nanosafety. In the present work, the complex phenomena occurring at the bio/nano interface were evaluated in a simple case study focusing on

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