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MilliporeSigma

05317

Millipore

Immobilon®-FL PVDF membrane

pore size 0.45 μm

Synonym(s):

Low fluorescent blotting membranes

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About This Item

UNSPSC Code:
41105339
NACRES:
NB.22

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size

10 cm × 10 cm

pore size

0.45 μm

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Chao Zhang et al.
Cell cycle (Georgetown, Tex.), 14(13), 2100-2108 (2015-05-06)
Osteosarcoma is the most common primary malignant bone tumor and affects a significant portion of pediatric oncology patients. Although surgery and adjuvant chemotherapy confer significant survival benefits, many patients go on to develop metastatic disease, particularly to the lungs, secondary
Hsin-Chieh Lan et al.
Toxicological sciences : an official journal of the Society of Toxicology, 148(1), 26-34 (2015-07-26)
Certain commonly used compounds that interfere with the functions of the endocrine system are classified as endocrine-disrupting chemicals (EDCs). Bisphenol A (BPA) is an EDC that is widely used in food containers. BPA levels in human sera are commonly observed

Articles

Ni-NTA-Atto Conjugates

Ni-NTA-Atto conjugates provide specific and highly sensitive detection of His-tagged fusion proteins.

Protocols

Introduction to Western Blot Detection of Proteins

Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.

Related Content

Questions

1–3 of 3 Questions  

  1. Is the PVDF membrane offered in rolls or sheets?

    1 answer

    1. This product is available as a 10cm x 10cm sheet. The membrane is also available in rolls of three lengths. Please see the link below to review these product options:
      https://www.sigmaaldrich.com/product/compare?term=Immobilon%C2%AE-FL%20PVDF%20membrane&focus=products&page=1&perpage=30&sort=relevance&type=product&products=IPFL85R%3AMM%3Asial%2CIPFL00005%3AMM%3Asial%2CIPFL00010%3AMM%3Asial

      Helpful?

  2. Can this PVDF membrane be used in Dot blot detecting nucleic acids?

    1 answer

    1. No, a positively charged membrane is required for nucleic acid blotting. At this time, a positively charged PVDF membrane is not available. Nylon membranes are often used for this application. Please see the links below to review products that may be of interest:

      Whatman Nytran N
      https://www.sigmaaldrich.com/substance/whatmannytrannnylonblottingmembranes1234598765

      Roche Membrane
      https://www.sigmaaldrich.com/product/roche/nylmro

      Blotting Nylon 66
      https://www.sigmaaldrich.com/product/sial/15356

      Helpful?

  3. The membrane LF (low fluorescence) to Western blot ...... Is it made of PVDF ?

    1 answer

    1. PVDF, as stated in the product name.

      Helpful?

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