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06699

Sigma-Aldrich

Atto 532

BioReagent, suitable for fluorescence, ≥90% (HPCE)

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MDL number:
NACRES:
NA.25

product line

BioReagent

Quality Level

assay

≥90% (HPCE)

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 533 nm; λem 560 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

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This Item
410518879340692
vibrant-m

06699

Atto 532

Essential+ Grade
vibrant-m

41051

Atto 488

-
vibrant-m

88793

Atto 532 NHS ester

-
vibrant-m

40692

Atto 532 DOPE

-
assay

≥90% (HPCE)

assay

≥90% (HPLC)

assay

≥90% (HPLC), ≥90% (degree of coupling)

assay

≥80.0% (HPCE)

fluorescence

λex 533 nm; λem 560 nm in 0.1 M phosphate pH 7.0

fluorescence

-

fluorescence

-

fluorescence

λex 537 nm; λem 559 nm±5 nm in ethanol

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

General description

Atto 532 is a new label with high molecular absorption (115,000) and quantum yield (0.90) as well as sufficient Stoke′s shift between excitation and emission maximum. It is optimized for excitation with frequency doubled Nd:YAG-Laser, and is characterized by high photostability.

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.
Atto 532 has been conjugated with the secondary antibodies for STED (stimulated emission depletion) microscopy and immunofluorescence studies.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Uffe V Schneider et al.
BMC biotechnology, 10, 4-4 (2010-01-28)
Melting temperature of DNA structures can be determined on the LightCycler using quenching of FAM. This method is very suitable for pH independent melting point (Tm) determination performed at basic or neutral pH, as a high throughput alternative to UV
3D reconstruction of high-resolution STED microscope images.
Punge A et al.
Microscopy Research and Technique, 71, 644-644 (2008)
John F Lesoine et al.
Nano letters, 12(6), 3273-3278 (2012-06-06)
We present a method for measuring the fluorescence from a single molecule hundreds of times without surface immobilization. The approach is based on the use of electroosmosis to repeatedly drive a single target molecule in a fused silica nanochannel through
Kiyoto Kamagata et al.
Journal of the American Chemical Society, 134(28), 11525-11532 (2012-06-14)
A method was developed to detect fluorescence intensity signals from single molecules diffusing freely in a capillary cell. A unique optical system based on a spherical mirror was designed to enable quantitative detection of the fluorescence intensity. Furthermore, "flow-and-stop" control
Moritz Marcinowski et al.
Nature structural & molecular biology, 18(2), 150-158 (2011-01-11)
The endoplasmic reticulum is the site of folding, assembly and quality control for proteins of the secretory pathway. The ATP-regulated Hsp70 chaperone BiP (heavy chain-binding protein), together with cochaperones, has important roles in all of these processes. The functional cycle

Articles

Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.

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