06713

Sigma-Aldrich

Atto 532 DMPE

suitable for fluorescence

Synonym(s):
1,2-Dimyristoyl-sn-glycero-3-phosphoethanolamine labeled with Atto 532
NACRES:
NA.32
Pricing and availability is not currently available.

Quality Level

assay

≥90.0% (HPCE)

fluorescence

λex 537 nm; λem 559 nm±5 nm in ethanol

suitability

suitable for fluorescence

storage temp.

−20°C

General description

Atto 532 is a fluorescent label related to the well-known dye Rhodamine 6G. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high photostability, and excellent water solubility. Thus Atto 532 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more. The fluorescence is excited most efficiently in the range 515 - 545 nm.
A suitable excitation source for Atto 532 is the 532 nm output of the frequency-doubled Nd:YAG laser.

Atto-Dye Labeled Phospholipids
Sigma-Aldrich offers a variety of glycero-phospholipids carrying one or two fatty acid groups (lipophilic groups) and a phosphate ester residue (hydrophilic group). They are labeled at the hydrophilic head group. After incorporation of the phospholipid into a membrane the fluorophore is located at the water/lipid interface of the membrane. We currently provide 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), palmitoyl-sn-glycero-phosphoethanolamine (PPE), and 1,2-dimyristoyl-sn-glycero-3-phospho-ethanolamine (DMPE) labeled with Atto-dyes.

find more information
here

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins.
Hofmann, M.; et al.
Proceedings of the National Academy of Sciences of the USA, 102(49), 17565?17569-17565?17569 (2005)
Technical Review. Types of Imaging-Direct STORM.
Jensen, E.; Crossman, D. J.
The Anatomical Record, 297(12), 2227-2231 (2014)
STED nanoscopy combined with optical tweezers reveals protein dynamics on densely covered DNA.
Heller, I.; et al.
Nature Methods, 10(9), 910-916 (2013)
Naked Dense Bodies Provoke Depression.
Hallermann, S.; et al.
The Journal of Neuroscience, 30(43), 14340-14345 (2010)
Self-Calibrated Line-Scan STED-FCS to Quantify Lipid Dynamics in Model and Cell Membranes.
Benda, A.; Ma, Y.; Gaus, K.
Biophysical Journal, 108(3), 596-609 (2015)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.