MilliporeSigma
All Photos(1)

06966

Sigma-Aldrich

Atto 655-Biotin

BioReagent, suitable for fluorescence, ≥90% (HPCE)

Synonym(s):
Biotin-Atto 655
NACRES:
NA.32

product line

BioReagent

Assay

≥90% (HPCE)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

transmittance

254 nm
655 nm

solubility

DMF: soluble
DMSO: soluble
acetonitrile: soluble

fluorescence

λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0

λ

in ethanol (with 0.1% trifluoroacetic acid: 655 nm ± 3 nm)

suitability

suitable for fluorescence

storage temp.

−20°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
286160163228923
Sigma-Aldrich

Sigma-Aldrich

06966

Atto 655-Biotin

Atto 425-Biotin BioReagent, suitable for fluorescence, ≥95.0% (HPCE)

Sigma-Aldrich

28616

Atto 425-Biotin

Atto 520-Biotin BioReagent, suitable for fluorescence, ≥90% (HPLC)

Sigma-Aldrich

01632

Atto 520-Biotin

Sigma-Aldrich

Sigma-Aldrich

28923

Atto 550-Biotin

assay

≥90% (HPCE)

assay

≥95.0% (HPCE)

assay

≥90% (HPLC)

assay

≥90% (HPLC)

form

powder

form

powder

form

powder

form

film

solubility

DMF: soluble, DMSO: soluble, acetonitrile: soluble

solubility

-

solubility

DMF: soluble, ethanol: soluble, methanol: soluble

solubility

-

fluorescence

λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 437 nm; λem 483 nm in 0.1 M phosphate pH 7.0

fluorescence

-

fluorescence

-

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

Application

Atto 655 conjugated to biotin is useful for binding to proteins (i.e., avidin and streptavidin). The fluorescent properties include excellent thermal and photo-stability, high quantum yield, strong absorption (663 nm) and fluorescence (684 nm), and low triplet formation. It is a zwitterionic dye, carrying a net electrical charge of zero. Electron donors such as guanine and tryptophan can easily quench the fluorescence of this fluorophore. Biotin conjugated with ATTO 655 was applied to assess efficiency of BSA/avidin structures bound to a surface.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 2

1 of 2

Biotin-4-Fluorescein

Sigma-Aldrich

B9431

Biotin-4-Fluorescein

Sigma-Aldrich

Sigma-Aldrich

07376

Atto 647 NHS ester

Bengang Xing et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 17(50), 14170-14177 (2011-11-16)
The molecular interactions of the glycopeptide antibiotic vancomycin (Van) with bacterial cell wall analogues N,N'-diacetyl-L-Lys-D-Ala-D-Ala (Ac(2) KdAdA) and N,N'-diacetyl-L-Lys-D-Ala-D-Lac (Ac(2) KdAdL) were investigated in neat water, phosphate buffer and HEPES buffer by using fluorescence correlation spectroscopy (FCS) and molecular dynamics
Zhixing Chen et al.
Journal of the American Chemical Society, 134(33), 13692-13699 (2012-08-10)
Chemical tags are now viable alternatives to fluorescent proteins for labeling proteins in living cells with organic fluorophores that have improved brightness and other specialized properties. Recently, we successfully rendered our TMP-tag covalent with a proximity-induced reaction between the protein
Fabian Heinemann et al.
Langmuir : the ACS journal of surfaces and colloids, 28(37), 13395-13404 (2012-08-16)
Fluorescence correlation spectroscopy (FCS) measurements are widely used for determination of diffusion coefficients of lipids and proteins in biological membranes. In recent years, several variants of FCS have been introduced. However, a comprehensive comparison of these methods on identical systems
Sridharan Rajagopalan et al.
Nucleic acids research, 39(6), 2294-2303 (2010-11-26)
The state of oligomerization of the tumor suppressor p53 is an important factor in its various biological functions. It has a well-defined tetramerization domain, and the protein exists as monomers, dimers and tetramers in equilibrium. The dissociation constants between oligomeric
Ryoji Abe et al.
Journal of the American Chemical Society, 133(43), 17386-17394 (2011-10-08)
Here, we describe a novel reagentless fluorescent biosensor strategy based on the antigen-dependent removal of a quenching effect on a fluorophore attached to antibody domains. Using a cell-free translation-mediated position-specific protein labeling system, we found that an antibody single chain

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service