MilliporeSigma
All Photos(1)

Documents

07376

Sigma-Aldrich

Atto 647 NHS ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

All Photos(1)
MDL number:
MFCD05865409
NACRES:
NA.25

product line

BioReagent

Quality Level

100

Assay

≥90% (HPLC)
≥90% (degree of coupling)

manufacturer/tradename

ATTO-TEC GmbH

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 646-652 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
003790874104022
Sigma-Aldrich

Sigma-Aldrich

07376

Atto 647 NHS ester

Atto 495 NHS ester BioReagent, suitable for fluorescence, ≥90% (HPLC)

Sigma-Aldrich

00379

Atto 495 NHS ester

Supelco

Supelco

08741

Atto 594 NHS ester

Sigma-Aldrich

Sigma-Aldrich

04022

Atto 665 NHS ester

assay

≥90% (HPLC), ≥90% (degree of coupling)

assay

≥90% (HPLC), ≥90% (degree of coupling)

assay

≥90% (HPLC), ≥90% (degree of coupling)

assay

≥90% (coupling to amines)

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

manufacturer/tradename

ATTO-TEC GmbH

manufacturer/tradename

ATTO-TEC GmbH

manufacturer/tradename

ATTO-TEC GmbH

manufacturer/tradename

ATTO-TEC GmbH

λ

in ethanol (with 0.1% trifluoroacetic acid)

λ

in acetonitrile (with 0.1% acetic acid)

λ

in ethanol (with 0.1% trifluoroacetic acid)

λ

-

Application

Atto 647 NHS ester is a reagent for the covalent labeling of molecules with Atto 647 flourescent dye. Atto 647 belongs to a new generation of fluorescent labels for the red spectral region. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high photostability, good water solubility, and very little triplet formation. Atto 647 is a zwitterionic dye with a net electrical charge of zero. Atto 647 is a pH sensitive product. While practically stable up to pH 8.5, it slowly degrades at higher pH.
Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 5

1 of 5

Atto 590 NHS ester BioReagent, suitable for fluorescence, ≥90.0% (degree of coupling)

Supelco

79636

Atto 590 NHS ester

Sigma-Aldrich

Sigma-Aldrich

88793

Atto 532 NHS ester

Sigma-Aldrich

Sigma-Aldrich

95349

Atto 647N amine

Sigma-Aldrich

Sigma-Aldrich

04022

Atto 665 NHS ester

Supelco

Supelco

02175

NTA - Atto 647 N

Breaking the Diffraction Barrier in Fluorescence Microscopy by Optical Shelving.
Bretschneider, S.; Eggeling, Chr.; Hell, S. W.
Physical Review Letters, 98(21) (2007)
Daniel Puntener et al.
Journal of virology, 85(1), 481-496 (2010-11-05)
Human adenoviruses (Ads) replicate and assemble particles in the nucleus. They organize a linear double-strand DNA genome into a condensed core with about 180 nucleosomes, by the viral proteins VII (pVII), pX, and pV attaching the DNA to the capsid.
Duncan Hill et al.
Biomedical microdevices, 13(4), 759-767 (2011-05-12)
This paper presents an overview of development of a novel disposable plastic biochip for multiplexed clinical diagnostic applications. The disposable biochip is manufactured using a low-cost, rapid turn- around injection moulding process and consists of nine parabolic elements on a
Laura Pastorino et al.
Journal of colloid and interface science, 357(1), 56-62 (2011-02-26)
The protein collagen is the major component of connective tissue and it is involved in many biological functions. Its degradation is at the basis of different pathological processes. The up-regulated expression of matrix metalloproteinases and the down-regulated expression of their
Glutamate-Induced AMPA Receptor Desensitization Increases Their Mobility and Modulates Short-Term Plasticity through Unbinding from Stargazin.
Constals, A., et al.
Neuron, 85(4), 707-803 (2015)
View All Related Papers

Articles

Peptide Labeling

Chromogenic and fluorogenic derivatives are invaluable tools for biochemistry, having numerous applications in enzymology, protein chemistry, immunology and histochemistry.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service